Rat ALCAM PicoKine ELISA Kit

Description: A sandwich quantitative ELISA assay kit for detection of Rat Activated Leukocyte Cell Adhesion Molecule (ALCAM) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Description: A sandwich quantitative ELISA assay kit for detection of Rat Activated Leukocyte Cell Adhesion Molecule (ALCAM) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Description: A sandwich quantitative ELISA assay kit for detection of Human Activated Leukocyte Cell Adhesion Molecule (ALCAM) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Description: A sandwich quantitative ELISA assay kit for detection of Human Activated Leukocyte Cell Adhesion Molecule (ALCAM) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Description: A sandwich quantitative ELISA assay kit for detection of Mouse Activated Leukocyte Cell Adhesion Molecule (ALCAM) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Description: A sandwich quantitative ELISA assay kit for detection of Mouse Activated Leukocyte Cell Adhesion Molecule (ALCAM) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Description: Description of target: Cell adhesion molecule that mediates both heterotypic cell-cell contacts via its interaction with CD6, as well as homotypic cell-cell contacts. Promotes T-cell activation and proliferation via its interactions with CD6 (By similarity). Contributes to the formation and maturation of the immunological synapse via its interactions with CD6 (By similarity). Mediates homotypic interactions with cells that express ALCAM. Mediates attachment of dendritic cells onto endothelial cells via homotypic interaction. Inhibits endothelial cell migration and promotes endothelial tube formation via homotypic interactions. Required for normal organization of the lymph vessel network. Required for normal hematopoietic stem cell engraftment in the bone marrow. Plays a role in hematopoiesis; required for normal numbers of hematopoietic stem cells in bone marrow. Promotes in vitro osteoblast proliferation and differentiation (By similarity). Promotes neurite extension, axon growth and axon guidance; axons grow preferentially on surfaces that contain ALCAM (By similarity). Mediates outgrowth and pathfinding for retinal ganglion cell axons (By similarity).;Species reactivity: Rat;Application: ;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.078 ng/mL

Description: Description of target: CD166 antigen is a 100-105 kD typeI transmembrane glycoprotein that is a member of the immunoglobulin superfamily of proteins. In humans it is encoded by the ALCAM gene. The human gene was mapped to chromosome 3q13.1-q13.2 by fluorescence in situ hybridization of cDNA probes to metaphase chromosomes. It is also called CD166 (cluster of differentiation 166), MEMD, SC-1/DM-GRASP/BEN in the chicken, and KG-CAM in the rat. This gene is expressed on activated T cells, activated monocytes, epithelial cells, fibroblasts, neurons, melanoma cells, and also in sweat and sebaceous glands. CD166 plays an important role in mediating adhesion interactions between thymic epithelial cells and CD6+ cells during intrathymic T cell development. Recently, CD166 has also been used as a potential cancer stem cell marker.;Species reactivity: Rat;Application: ELISA;Assay info: ;Sensitivity: <10pg/ml

Description: Enzyme-linked immunosorbent assay kit for quantification of Rat ALCAM in samples from serum, plasma, tissue homogenates and other biological fluids.

Description: For quantitative detection of rat VEGF in cell culture supernates, serum and plasma(heparin, EDTA, citrate).

Description: For quantitative detection of rat CXCL1 in cell culture supernates, serum and plasma(EDTA).

Description: For quantitative detection of rat TrkA in cell culture supernates and cell lysates.

Description: For quantitative detection of rat KIM1 in cell culture supernates, serum, plasma(heparin, EDTA) and urine.

Description: For quantitative detection of rat Endothelin in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).

Description: For quantitative detection of rat EGF in cell culture supernates, serum, plasma(heparin, EDTA)and urine.

Description: For quantitative detection of rat RAGE in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).

Description: For quantitative detection of rat CRP in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).

Description: For quantitative detection of rat EPO in cell culture supernates, serum and plasma(heparin).

Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Rattus;Sensitivity: 0.094 ng/ml

Description: For quantitative detection of rat TIMP-1 in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).

Description: For quantitative detection of rat IL-18 in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).

Description: For quantitative detection of rat MMP-2 in cell culture supernates, serum and plasma(heparin).

Description: For Quantitative Detection of rat CXCL7 in cell culture supernates, serum and plasma (heparin, EDTA).

Description: For quantitative detection of rat CXCL5 in cell culture supernates, serum and plasma(heparin, EDTA, citrate).

Description: For quantitative detection of rat IL-13 in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).

Description: For quantitative detection of rat soluble E-Selectin in cell culture supernates, serum and plasma(heparin, citrate).

Description: For quantitative detection of rat soluble L-Selectin in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).

Description: ELISA Kit for detection of ALCAM in the research laboratory

Description: The Fast version of Picokine ELISA kits, assay takes less than 1.5 hours. Detect Rat Fibronectin/FN1 with <15pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, serum and plasma(heparin, EDTA, citrate). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Fibronectin/FN1 tissue specificity: Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix.

Description: The Fast version of Picokine ELISA kits, assay takes less than 1.5 hours. Detect Rat GDNF with <4pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. GDNF tissue specificity:

Description: The Fast version of Picokine ELISA kits, assay takes less than 1.5 hours. Detect Rat Rank/TNFRSF11A with <10pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates and serum. This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Rank/TNFRSF11A tissue specificity:

Description: The Fast version of Picokine ELISA kits, assay takes less than 1.5 hours. Detect Rat Adiponectin/ADIPOQ with <10pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, serum, plasma(heparin, EDTA)and urine. This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Adiponectin/ADIPOQ tissue specificity:

Description: The Fast version of Picokine ELISA kits, assay takes less than 1.5 hours. Detect Rat Cd44 with <10pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Cd44 tissue specificity:

Description: For quantitative detection of rat CD80 in cell culture supernates and serum.

Description: For quantitative detection of rat CD86 in cell culture supernates and serum.

Description: For quantitative detection of rat MIP-2 in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).

Description: For quantitative detection of rat NGAL in cell culture supernates, serum, plasma(heparin) and urine.

Description: For quantitative detection of rat MCP-1 in cell culture supernates and serum.

Description: For quantitative detection of activated rat TGF-beta 2 in cell culture supernates, serum and plasma(heparin, EDTA, citrate).

Description: Description of target: ;Species reactivity: Bovine;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.078ng/mL

Description: Description of target: ;Species reactivity: Chicken;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.15ng/mL

Description: Description of target: ;Species reactivity: Dog;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.078ng/mL

Description: Description of target: Cell adhesion molecule that mediates both heterotypic cell-cell contacts via its interaction with CD6, as well as homotypic cell-cell contacts (PubMed:7760007, PubMed:15496415, PubMed:15048703, PubMed:16352806, PubMed:23169771, PubMed:24945728). Promotes T-cell activation and proliferation via its interactions with CD6 (PubMed:15048703, PubMed:16352806, PubMed:24945728). Contributes to the formation and maturation of the immunological synapse via its interactions with CD6 (PubMed:15294938, PubMed:16352806). Mediates homotypic interactions with cells that express ALCAM (PubMed:15496415, PubMed:16352806). Required for normal hematopoietic stem cell engraftment in the bone marrow (PubMed:24740813). Mediates attachment of dendritic cells onto endothelial cells via homotypic interaction (PubMed:23169771). Inhibits endothelial cell migration and promotes endothelial tube formation via homotypic interactions (PubMed:15496415, PubMed:23169771). Required for normal organization of the lymph vessel network. Required for normal hematopoietic stem cell engraftment in the bone marrow. Plays a role in hematopoiesis; required for normal numbers of hematopoietic stem cells in bone marrow. Promotes in vitro osteoblast proliferation and differentiation (By similarity). Promotes neurite extension, axon growth and axon guidance; axons grow preferentially on surfaces that contain ALCAM. Mediates outgrowth and pathfinding for retinal ganglion cell axons (By similarity).By similarity <p>Manually curated information which has been propagated from a related experimentally characterized protein.</p> <p><a href="/manual/evidences#ECO:0000250">More…</a></p> Manual assertion inferred from sequence similarity toiUniProtKB:P42292 (CD166_CHICK)6 Publications <p>Manually curated information for which there is published experimental evidence.</p> <p><a href="/manual/evidences#ECO:0000269">More…</a></p> Manual assertion based on experiment iniRef.1"Cloning, mapping, and characterization of activated leukocyte-cell adhesion molecule (ALCAM), a CD6 ligand."_x005F_x005F_x000D_Bowen M.A., Patel D.D., Li X., Modrell B., Malacko A.R., Wang W.-C., Marquardt H., Neubauer M., Pesando J.M., Francke U., Haynes B.F., Aruffo A._x005F_x005F_x000D_J. Exp. Med. 181:2213-2220(1995) [PubMed] [Europe PMC] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANT MET-301, PARTIAL PROTEIN SEQUENCE, INTERACTION WITH CD6, SUBUNIT, SUBCELLULAR LOCATION, FUNCTION, TISSUE SPECIFICITY.Ref.2"Molecular isolation and characterization of a soluble isoform of activated leukocyte cell adhesion molecule that modulates endothelial cell function."_x005F_x005F_x000D_Ikeda K., Quertermous T._x005F_x005F_x000D_J. Biol. Chem. 279:55315-55323(2004) [PubMed] [Europe PMC] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3), SUBCELLULAR LOCATION (ISOFORM 3), FUNCTION (ISOFORMS 1 AND 3), GLYCOSYLATION, TISSUE SPECIFICITY.Ref.10"Frontline: Optimal T cell activation requires the engagement of CD6 and CD166."_x005F_x005F_x000D_Hassan N.J., Barclay A.N., Brown M.H._x005F_x005F_x000D_Eur. J. Immunol. 34:930-940(2004) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION, INTERACTION WITH CD6, SUBUNIT, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.Ref.11"Relevance of CD6-mediated interactions in T cell activation and proliferation."_x005F_x005F_x000D_Gimferrer I., Calvo M., Mittelbrunn M., Farnos M., Sarrias M.R., Enrich C., Vives J., Sanchez-Madrid F., Lozano F._x005F_x005F_x000D_J. Immunol. 173:2262-2270(2004) [PubMed] [Europe PMC] [Abstract]Cited for: SUBCELLULAR LOCATION.Ref.13"Long-term engagement of CD6 and ALCAM is essential for T-cell proliferation induced by dendritic cells."_x005F_x005F_x000D_Zimmerman A.W., Joosten B., Torensma R., Parnes J.R., van Leeuwen F.N., Figdor C.G._x005F_x005F_x000D_Blood 107:3212-3220(2006) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH CD6, SUBUNIT, TISSUE SPECIFICITY.Ref.20"Modulation of CD6 function through interaction with galectin-1 and -3."_x005F_x005F_x000D_Escoda-Ferran C., Carrasco E., Caballero-Banos M., Miro-Julia C., Martinez-Florensa M., Consuegra-Fernandez M., Martinez V.G., Liu F.T., Lozano F._x005F_x005F_x000D_FEBS Lett. 588:2805-2813(2014) [PubMed] [Europe PMC] [Abstract]Cited for: INTERACTION WITH CD6; LGALS1 AND LGALS3, GLYCOSYLATION, FUNCTION, SUBCELLULAR LOCATION. Isoform 3: Inhibits activities of membrane-bound isoforms by competing for the same interaction partners. Inhibits cell attachment via homotypic interactions. Promotes endothelial cell migration. Inhibits endothelial cell tube formation.1 Publication <p>Manually curated information for which there is published experimental evidence.</p> <p><a href="/manual/evidences#ECO:0000269">More…</a></p> Manual assertion based on experiment iniRef.2"Molecular isolation and characterization of a soluble isoform of activated leukocyte cell adhesion molecule that modulates endothelial cell function."_x005F_x005F_x000D_Ikeda K., Quertermous T._x005F_x005F_x000D_J. Biol. Chem. 279:55315-55323(2004) [PubMed] [Europe PMC] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3), SUBCELLULAR LOCATION (ISOFORM 3), FUNCTION (ISOFORMS 1 AND 3), GLYCOSYLATION, TISSUE SPECIFICITY. ;Species reactivity: Human;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich Immunoassay;Sensitivity: < 0.068 ng/mL

Description: Description of target: CD166 antigen is a 100-105 kD typeI transmembrane glycoprotein that is a member of the immunoglobulin superfamily of proteins. In humans it is encoded by the ALCAM gene. The gene was mapped to human chromosome 3q13.1-q13.2 by fluorescence in situ hybridization of cDNA probes to metaphase chromosomes. It is also called CD166 (cluster of differentiation 166), MEMD, SC-1/DM-GRASP/BEN in the chicken, and KG-CAM in the rat. It is expressed on activated T cells, activated monocytes, epithelial cells, fibroblasts, neurons, melanoma cells, and also in sweat and sebaceous glands. CD166 protein expression is reported to be upregulated in a cell line deriving from a metastasizing melanoma. CD166 plays an important role in mediating adhesion interactions between thymic epithelial cells and CD6+ cells during intrathymic T cell development. Recently, CD166 has also been used as a potential cancer stem cell marker.;Species reactivity: Human;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich Immunoassay;Sensitivity: <= 10 pg/mL

Description: Description of target: CD166 antigen is a 100-105 kD typeI transmembrane glycoprotein that is a member of the immunoglobulin superfamily of proteins. In humans it is encoded by the ALCAM gene. The gene was mapped to human chromosome 3q13.1-q13.2 by fluorescence in situ hybridization of cDNA probes to metaphase chromosomes. It is also called CD166 (cluster of differentiation 166), MEMD, SC-1/DM-GRASP/BEN in the chicken, and KG-CAM in the rat. It is expressed on activated T cells, activated monocytes, epithelial cells, fibroblasts, neurons, melanoma cells, and also in sweat and sebaceous glands. CD166 protein expression is reported to be upregulated in a cell line deriving from a metastasizing melanoma. CD166 plays an important role in mediating adhesion interactions between thymic epithelial cells and CD6+ cells during intrathymic T cell development. Recently, CD166 has also been used as a potential cancer stem cell marker.;Species reactivity: Mouse;Application: ELISA;Assay info: ;Sensitivity: <10pg/ml

Description: Enzyme-linked immunosorbent assay kit for quantification of Human ALCAM in samples from serum, plasma, tissue homogenates and other biological fluids.

Description: Enzyme-linked immunosorbent assay kit for quantification of Mouse ALCAM in samples from serum, plasma, tissue homogenates and other biological fluids.

Description: For quantitative detection of human REG3A in cell culture supernates, serum and plasma (heparin).

Description: For quantitative detection of human SLITRK2 in cell culture supernates, serum and plasma (heparin).

Description: For quantitative detection of human CLEC1B in cell culture supernates, serum and plasma (heparin, EDTA).

Description: For quantitative detection of human CA4 in cell culture supernates, serum and plasma (heparin, EDTA, citrate).

Description: For quantitative detection of human CNDP1 in cell culture supernates, serum and plasma (heparin, EDTA).

Description: For quantitative detection of human TRAIL in cell culture supernates, cell lysates, serum, plasma(heparin, EDTA) and saliva.

Description: For quantitative detection of human uPAR in cell culture supernates, serum, plasma(heparin, EDTA) and urine.

Description: For quantitative detection of human VEGF in cell culture supernates, serum and plasma(heparin, EDTA, citrate).

Description: For quantitative detection of mouse VEGF in cell culture supernates, serum and plasma(heparin, EDTA, citrate).

Description: For quantitative detection of human ACE in cell culture supernates, serum, plasma(heparin) and saliva.

Description: For quantitative detection of mouse ACE in cell culture supernates, serum and plasma(heparin).

Description: For quantitative detection of human CCL1 in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).

Description: For quantitative detection of mouse CD30 in cell culture supernates and serum.

Description: For quantitative detection of mouse CD30L in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).

Description: For quantitative detection of human soluble CD40L in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).

Description: For quantitative detection of mouse CD40L in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).

Description: For quantitative detection of mouse LIF in cell culture supernates, serum and plasma (heparin, EDTA).

Description: For quantitative detection of human Resistin in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).

Description: For quantitative detection of mouse Resistin in cell culture supernates, serum and plasma(heparin).

Description: For quantitative detection of human Prolactin in cell culture supernates, serum and plasma(heparin, citrate).

Description: For quantitative detection of mouse Prolactin in cell culture supernates, serum and plasma(heparin, citrate).

Description: For quantitative detection of human Adiponectin in cell culture supernates, serum, plasma(heparin, EDTA)and urine.