Mouse Osteoactivin/GPNMB PicoKine ELISA Kit

Description: Description of target: Transmembrane glycoprotein NMB is a protein that is encoded by the GPNMB gene. In osteoblast progenitor cells, GPNMB works as a positive regulator of osteoblast differentiation during later stages of matrix maturation and mineralization that is mediated at least in part by BMP-2 in a SMAD1 dependent manner to promote osteoblast differentiation. GPNMB can enhance the repairing process in bone fracture, demonstrated by its high expression during chondrogenesis (soft callus) and osteogenesis (hard callus) compared to the intact femurs that is why Osteoactivin (OA) could be a novel therapeutic agent used to treat generalized osteoporosis or localized osteopenia during fracture repair by stimulating bone growth and regeneration. Similarly, GPNMB expression increases during osteoclast differentiation and it is functionally implicated in this process, possibly by promoting the fusion of osteoclast progenitor cells.;Species reactivity: Mouse;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich Immunoassay;Sensitivity: <= 10 pg/mL

Description: Enzyme-linked immunosorbent assay kit for quantification of Mouse GPNMB/Osteoactivin in samples from serum, plasma, tissue homogenates and other biological fluids.

Description: Enzyme-linked immunosorbent assay kit for quantification of Human GPNMB/Osteoactivin in samples from serum, plasma, tissue homogenates and other biological fluids.

Description: Description of target: Transmembrane glycoprotein NMB is a protein that in humans is encoded by the GPNMB gene. In osteoblast progenitor cells, GPNMB works as a positive regulator of osteoblast differentiation during later stages of matrix maturation and mineralization that is mediated at least in part by BMP-2 in a SMAD1 dependent manner to promote osteoblast differentiation. GPNMB can enhance the repairing process in bone fracture, demonstrated by its high expression during chondrogenesis (soft callus) and osteogenesis (hard callus) compared to the intact femurs that is why Osteoactivin (OA) could be a novel therapeutic agent used to treat generalized osteoporosis or localized osteopenia during fracture repair by stimulating bone growth and regeneration. Similarly, GPNMB expression increases during osteoclast differentiation and it is functionally implicated in this process, possibly by promoting the fusion of osteoclast progenitor cells.;Species reactivity: Human;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich Immunoassay;Sensitivity: <= 10 pg/mL

Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.2.

Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.2.

Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.2.

Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.2.

Description: The Human Osteoactivin ELISA KIT is suitable for the quantitative determination of human soluble Osteoactivin/GPNMB concentrations in cell culture supernates, serum, and plasma.

Description: Osteoactivin is an intracellular glycoprotein belongs to the NMB/pMEL-17 family, which is asscociated with cell endosomal/lysomal compartments. Human Osteoactivin is a 560 amino acid type I transmembrane protein, and one alternate splice form shows a 12 amino acid insert between amino acid 339-340. An additional 206 amino acid isoform shows a mutation at position 181 that results in a 26 amino acid substitution for the C-terminal 380 amino acids. Cells knowns to express Osteoactivin include fibroblast, osteoblasts, myeloid dendritic cell, melanocytes, plus fetal chondrocytes and stratum basale keratinocytes, macrophages/keratinocytes.

Description: Transmembrane glycoprotein NMB (GPNMB) is also known as Transmembrane glycoprotein HGFIN, DC-HIL and Osteoactivin (OA), which belongs to the PMEL/NMB family. GPNMB contains one PKD domain. GPNMB is a transmembrane glycoprotein that is up-regulated in various cancer cells, including in glioblastoma multiforme and is expressed in many melanoma cells, as well as in tissue macrophages. GPNMB protein acts as a downstream mediator of BMP-2 effects on osteoblast differentiation and function. GPNMB participates in bone mineralization, and functions as a negative regulator of inflammation in macrophages.

Description: Human Osteoactivin protein, His tag, was expressed in human 293 cells. (Uniprot ID: AAH32783)

Description: Human Osteoactivin protein, His tag, was expressed in human 293 cells. (Uniprot ID: AAH32783)

Description: For quantitative detection of mouse Osteoadherin in cell culture supernates, serum and plasma (heparin, EDTA).

Description: For quantitative detection of human Osteoadherin in cell culture supernates, serum and plasma (heparin, EDTA).

Description: The Fast version of Picokine ELISA kits, assay takes less than 1.5 hours. Detect Mouse Osteoprotegerin/TNFRSF11B with <5pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, serum and plasma(EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Osteoprotegerin/TNFRSF11B tissue specificity: Highly expressed in liver, lung, stomach, intestines and calvaria. Highly expressed in decidua and placenta, and in embryo.

Description: For quantitative detection of mouse MPO in cell culture supernates, cell lysates, serum and plasma(heparin, EDTA).

Description: For quantitative detection of mouse CRP in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).

Description: For quantitative detection of mouse SAA in cell culture supernates, serum, plasma(heparin, EDTA) and urine.

Description: For quantitative detection of mouse HGF in cell culture supernates, serum and plasma(EDTA, citrate).