Mouse S100A9 PicoKine ELISA Kit

Description: A sandwich quantitative ELISA assay kit for detection of Mouse S100 Calcium Binding Protein A9 (S100A9) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Description: A sandwich quantitative ELISA assay kit for detection of Mouse S100 Calcium Binding Protein A9 (S100A9) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Description: A sandwich quantitative ELISA assay kit for detection of Bovine S100 Calcium Binding Protein A9 (S100A9) in samples from serum, plasma or other biological fluids.

Description: A sandwich quantitative ELISA assay kit for detection of Bovine S100 Calcium Binding Protein A9 (S100A9) in samples from serum, plasma or other biological fluids.

Description: A sandwich quantitative ELISA assay kit for detection of Human S100 Calcium Binding Protein A9 (S100A9) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Description: A sandwich quantitative ELISA assay kit for detection of Human S100 Calcium Binding Protein A9 (S100A9) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Description: Description of target: S100A9 is a calcium- and zinc-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. It can induce neutrophil chemotaxis, adhesion, can increase the bactericidal activity of neutrophils by promoting phagocytosis via activation of SYK, PI3K/AKT, and ERK1/2 and can induce degranulation of neutrophils by a MAPK-dependent mechanism. Predominantly found as calprotectin (S100A8/A9) which has a wide plethora of intra- and extracellular functions. The intracellular functions include: facilitating leukocyte arachidonic acid trafficking and metabolism, modulation of the tubulin-dependent cytoskeleton during migration of phagocytes and activation of the neutrophilic NADPH-oxidase. Activates NADPH-oxidase by facilitating the enzyme complex assembly at the cell membrane, transferring arachidonic acid, an essential cofactor, to the enzyme complex and S100A8 contributes to the enzyme assembly by directly binding to NCF2/P67PHOX. The extracellular functions involve proinfammatory, antimicrobial, oxidant-scavenging and apoptosis-inducing activities. Its proinflammatory activity includes recruitment of leukocytes, promotion of cytokine and chemokine production, and regulation of leukocyte adhesion and migration. Acts as an alarmin or a danger associated molecular pattern (DAMP) molecule and stimulates innate immune cells via binding to pattern recognition receptors such as Toll-like receptor 4 (TLR4) and receptor for advanced glycation endproducts (AGER). Binding to TLR4 and AGER activates the MAP-kinase and NF-kappa-B signaling pathways resulting in the amplification of the proinflammatory cascade. Has antimicrobial activity towards bacteria and fungi and exerts its antimicrobial activity probably via chelation of Zn2+ which is essential for microbial growth. Can induce cell death via autophagy and apoptosis and this occurs through the cross-talk of mitochondria and lysosomes via reactive oxygen species (ROS) and the process involves BNIP3. Can regulate neutrophil number and apoptosis by an anti-apoptotic effect; regulates cell survival via ITGAM/ITGB and TLR4 and a signaling mechanism involving MEK-ERK. Its role as an oxidant scavenger has a protective role in preventing exaggerated tissue damage by scavenging oxidants. The iNOS-S100A8/A9 transnitrosylase complex is proposed to direct selective inflammatory stimulus-dependent S-nitrosylation of multiple targets such as GAPDH, NXA5, EZR, MSN and VIM by recognizing a [IL]-x-C-x-x-[DE] motif (By similarity).By similarity <p>Manually curated information which has been propagated from a related experimentally characterized protein.</p> <p><a href="/manual/evidences#ECO:0000250">More…</a></p> Manual assertion inferred from sequence similarity toiUniProtKB:P06702 (S10A9_HUMAN)6 Publications <p>Manually curated information for which there is published experimental evidence.</p> <p><a href="/manual/evidences#ECO:0000269">More…</a></p> Manual assertion based on experiment iniRef.6"MRP8 and MRP14 control microtubule reorganization during transendothelial migration of phagocytes."_x005F_x005F_x000D_Vogl T., Ludwig S., Goebeler M., Strey A., Thorey I.S., Reichelt R., Foell D., Gerke V., Manitz M.P., Nacken W., Werner S., Sorg C., Roth J._x005F_x005F_x000D_Blood 104:4260-4268(2004) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION, INTERACTION WITH TUBULIN, PHOSPHORYLATION.Ref.8"Mrp8 and Mrp14 are endogenous activators of Toll-like receptor 4, promoting lethal, endotoxin-induced shock."_x005F_x005F_x000D_Vogl T., Tenbrock K., Ludwig S., Leukert N., Ehrhardt C., van Zoelen M.A.D., Nacken W., Foell D., van der Poll T., Sorg C., Roth J._x005F_x005F_x000D_Nat. Med. 13:1042-1049(2007) [PubMed] [Europe PMC] [Abstract]Cited for: DISRUPTION PHENOTYPE, FUNCTION, SUBCELLULAR LOCATION, SUBUNIT, INTERACTION WITH TLR4 AND LY96.Ref.9"S100A8 and S100A9 mediate endotoxin-induced cardiomyocyte dysfunction via the receptor for advanced glycation end products."_x005F_x005F_x000D_Boyd J.H., Kan B., Roberts H., Wang Y., Walley K.R._x005F_x005F_x000D_Circ. Res. 102:1239-1246(2008) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION, INTERACTION WITH ATP2A2 AND AGER.Ref.11"Identification of human S100A9 as a novel target for treatment of autoimmune disease via binding to quinoline-3-carboxamides."_x005F_x005F_x000D_Bjoerk P., Bjoerk A., Vogl T., Stenstroem M., Liberg D., Olsson A., Roth J., Ivars F., Leanderson T._x005F_x005F_x000D_PLoS Biol. 7:E97-E97(2009) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION, SUBCELLULAR LOCATION, SUBUNIT, INTERACTION WITH TLR4; LY96 AND AGER, QUINOLINE-3-CARBOXAMIDE BINDING.Ref.14"S100A9 differentially modifies phenotypic states of neutrophils, macrophages, and dendritic cells: implications for atherosclerosis and adipose tissue inflammation."_x005F_x005F_x000D_Averill M.M., Barnhart S., Becker L., Li X., Heinecke J.W., Leboeuf R.C., Hamerman J.A., Sorg C., Kerkhoff C., Bornfeldt K.E._x005F_x005F_x000D_Circulation 123:1216-1226(2011) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION.Ref.15"Induction of nuclear factor-kappaB responses by the S100A9 protein is Toll-like receptor-4-dependent."_x005F_x005F_x000D_Riva M., Kaellberg E., Bjoerk P., Hancz D., Vogl T., Roth J., Ivars F., Leanderson T._x005F_x005F_x000D_Immunology 137:172-182(2012) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION. ;Species reactivity: Mouse;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich Immunoassay;Sensitivity: < 0.056 ng/mL

Description: Description of target: ;Species reactivity: Mouse;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.059 ng/mL

Description: Description of target: Protein S100A9 also known as migration inhibitory factor-related protein 14 (MRP-14) or calgranulin-B is a protein that is encoded by the S100A9 gene. S100A9 is a member of the S100 family of proteins containing 2 EF hand calcium-binding motifs. It is mapped to Chromosome 3 in mouse. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. This protein may function in the inhibition of casein kinase. S100A9 complexes with S100A8, another member of the S100 family of calcium-modulated proteins regulates myeloid cell function by binding to Toll-like receptor-4 (TLR-4) and the receptor for advanced glycation end products.;Species reactivity: Mouse;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich Immunoassay;Sensitivity: <= 10 pg/mL

Description: Description of target: S100A9 is a calcium- and zinc-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. It can induce neutrophil chemotaxis, adhesion, can increase the bactericidal activity of neutrophils by promoting phagocytosis via activation of SYK, PI3K/AKT, and ERK1/2 and can induce degranulation of neutrophils by a MAPK-dependent mechanism. Predominantly found as calprotectin (S100A8/A9) which has a wide plethora of intra- and extracellular functions. The intracellular functions include: facilitating leukocyte arachidonic acid trafficking and metabolism, modulation of the tubulin-dependent cytoskeleton during migration of phagocytes and activation of the neutrophilic NADPH-oxidase. Activates NADPH-oxidase by facilitating the enzyme complex assembly at the cell membrane, transferring arachidonic acid, an essential cofactor, to the enzyme complex and S100A8 contributes to the enzyme assembly by directly binding to NCF2/P67PHOX. The extracellular functions involve proinfammatory, antimicrobial, oxidant-scavenging and apoptosis-inducing activities. Its proinflammatory activity includes recruitment of leukocytes, promotion of cytokine and chemokine production, and regulation of leukocyte adhesion and migration. Acts as an alarmin or a danger associated molecular pattern (DAMP) molecule and stimulates innate immune cells via binding to pattern recognition receptors such as Toll-like receptor 4 (TLR4) and receptor for advanced glycation endproducts (AGER). Binding to TLR4 and AGER activates the MAP-kinase and NF-kappa-B signaling pathways resulting in the amplification of the proinflammatory cascade. Has antimicrobial activity towards bacteria and fungi and exerts its antimicrobial activity probably via chelation of Zn2+ which is essential for microbial growth. Can induce cell death via autophagy and apoptosis and this occurs through the cross-talk of mitochondria and lysosomes via reactive oxygen species (ROS) and the process involves BNIP3. Can regulate neutrophil number and apoptosis by an anti-apoptotic effect; regulates cell survival via ITGAM/ITGB and TLR4 and a signaling mechanism involving MEK-ERK. Its role as an oxidant scavenger has a protective role in preventing exaggerated tissue damage by scavenging oxidants. The iNOS-S100A8/A9 transnitrosylase complex is proposed to direct selective inflammatory stimulus-dependent S-nitrosylation of multiple targets such as GAPDH, NXA5, EZR, MSN and VIM by recognizing a.;Species reactivity: Mouse;Application: ;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 31.31 pg/mL

Description: For quantitative detection of mouse PLTP in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).

Description: For quantitative detection of mouse Hemojuvelin in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).

Description: For quantitative detection of mouse Endostatin in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).

Description: For quantitative detection of mouse FGF21 in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).

Description: For quantitative detection of mouse Epiregulin in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).

Description: For quantitative detection of Mouse Chordin in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).

Description: For quantitative detection of activated mouse CD5L in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).

Description: For quantitative detection of mouse CD44 in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).

Description: For quantitative detection of mouse EGFR in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).

Description: For quantitative detection of activated mouse EPCR in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).

Description: For quantitative detection of mouse IDS in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).

Description: For quantitative detection of mouse FABP4 in cell culture supernates, serum, plasma(heparin, EDTA) and cell lysates.

Description: For quantitative detection of mouse Persephin in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).

Description: For quantitative detection of mouse GDF5 in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).

Description: For quantitative detection of mouse TREML1 in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).

Description: For quantitative detection of mouse WIF1 in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).

Description: For quantitative detection of mouse GREM2 in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).

Description: For Quantitative Detection of mouse Artemin in cell culture supernates, serum and plasma (heparin, EDTA).

Description: For quantitative detection of mouse LDLR in cell culture supernates, serum, plasma(heparin) and urine.

Description: For quantitative detection of mouse BCAM in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).

Description: For quantitative detection of mouse S100A13 in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).

Description: For quantitative detection of mouse DKK4 in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).

Description: For quantitative detection of mouse KLK1 in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).

Description: For quantitative detection of mouse CA9 in cell culture supernates, serum, plasma (heparin, EDTA, citrate) and urine.

Description: For Quantitative Detection of mouse Mimecan in cell culture supernates, serum and plasma (heparin, EDTA).

Description: For Quantitative Detection of mouse PGLYRP1 in cell culture supernates, serum and plasma (heparin, EDTA).

Description: For Quantitative Detection of mouse TFF1 in cell culture supernates, serum and plasma (heparin, EDTA).

Description: For Quantitative Detection of mouse PON1 in cell culture supernates, serum and plasma(heparin).

Description: For Quantitative Detection of mouse FABP2 in cell culture supernates, serum and plasma (heparin, EDTA).