GMP-grade neural progenitor derivation and differentiation from clinical-grade human embryonic stem cells

cromauv.org

GMP-grade neural progenitor derivation and differentiation from clinical-grade human embryonic stem cells

Background: A serious problem for the medical use of human pluripotent stem cells is the event of secure, sturdy and managed differentiation protocols. Adaptation of analysis protocols utilizing reagents designated as research-only to these that are appropriate for medical use, also known as good manufacturing follow (GMP) reagents, is an important and laborious step within the translational pipeline. Nonetheless, revealed protocols to help this course of stay very restricted.

Strategies: We tailored research-grade protocols for the derivation and differentiation of long-term neuroepithelial stem cell progenitors (lt-NES) to GMP-grade reagents and elements appropriate for medical functions. We screened the robustness of the protocol with six clinical-grade hESC traces deposited within the UK Stem Cell Financial institution.

Outcomes: Right here, we current a brand new GMP-compliant protocol to derive lt-NES, that are multipotent, bankable and karyotypically steady. This protocol resulted in sturdy and reproducible differentiation of a number of clinical-grade embryonic stem cells from which we derived lt-NES. Moreover, GMP-derived lt-NES demonstrated a excessive neurogenic potential whereas retaining the flexibility to be redirected to a number of neuronal sub-types.

Conclusions: Total, we report the feasibility of derivation and differentiation of clinical-grade embryonic stem cell traces into lt-NES beneath GMP-compliant circumstances. Our protocols may very well be used as a versatile software to hurry up translation-to-clinic of pluripotent stem cells for a wide range of neurological therapies or regenerative medication research.

cromauv.org

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Individual Reaction Mix

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G065-8 200 reactions
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MINI TUBE, INDIVIDUAL, POLYPROPYLENE, 0.65 ML, CLEAR, NONSTERILE, BULK

MTS-06-C 960/pk
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Description: Deep Well/Assay Plates - Axygen; Assay/Other - Axygen

MINI TUBE, INDIVIDUAL, POLYPROPYLENE, 1.1 ML, CLEAR, NONSTERILE, BULK

MTS-11-C 960/pk
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Description: Deep Well/Assay Plates - Axygen; Assay/Other - Axygen

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MINI TUBE, INDIVIDUAL, POLYPROPYLENE, 0.65 ML, CLEAR, RACKED, STERILE, BULK

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EUR 517.2
Description: Deep Well/Assay Plates - Axygen; Assay/Other - Axygen

MINI TUBE, INDIVIDUAL, POLYPROPYLENE, 1.1 ML, CLEAR, RACKED, STERILE, BULK

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EUR 397.2
Description: Deep Well/Assay Plates - Axygen; Assay/Other - Axygen

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Dynamic Laser Speckle Imaging meets Machine Studying to allow Fast Antibacterial Susceptibility Testing (DyRAST)

Fast antibacterial susceptibility testing (RAST) strategies which measure change of a bacterial phenotype in response to a given therapy are of great significance in healthcare, as they’ll help care-givers within the well timed administration of appropriate remedies. Numerous RAST strategies have been reported for monitoring bacterial phenotypes, together with dimension, form, movement, and redox state. Nonetheless, they nonetheless require cumbersome and costly devices – which hinders their software in resource-limited environments – and/or make the most of labeling reagents which may intervene with antibiotics and add to price.

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Polymer-Supported Phosphoric, Phosphonic and Phosphinic Acids-From Synthesis to Properties and Purposes in Separation Processes

Environment friendly separation applied sciences are essential to the atmosphere and world financial system. The problem posed to scientists is how one can engineer selectivity in the direction of a focused substrate, particularly from multicomponent options. Polymer-supported reagents have gained plenty of consideration on this context, as they eradicate plenty of inconveniences regarding broadly used solvent extraction strategies.

However, the selection of an applicable ligand for immobilization could also be derived from the habits of soluble compounds beneath solvent extraction circumstances. Organophosphorus compounds play a big function in separation science and expertise. The options they possess, equivalent to variable oxidation states, multivalence, asymmetry and metal-binding properties, spotlight their standing as a novel and versatile class of compounds, able to selective separations continuing by means of completely different mechanisms.

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G065-7 200 reactions
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G065-8 200 reactions
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Individual Standard Mix A for 3/90 and 7/91 SOW - 1ML

CLPP-A90 1ML
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0.2ml Individual PCR Tubes

24-153 Bag of 1000 Tubes/Unit
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24-178 Bag of 1000 Tubes/Unit
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Description: Flat Cap, Natural

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PCR1186 PK1000
EUR 63.36

0.2ml Individual PCR Tubes - PK1000

PCR1178 PK1000
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Individual Storage Plate Caps (960)

AB-1179 each
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INDIVIDUAL ADAPTERS FOR 0.5ML TUBES,1/6

480134 6/pk
EUR 75.6
Description: Lab Equipment; General Purpose Centrifuges (affliated brand)

INDIVIDUAL ADAPTERS FOR 0.2ML TUBES,1/6

480135 6/pk
EUR 75.6
Description: Lab Equipment; General Purpose Centrifuges (affliated brand)

RNA Cleanup Kit - Individual Packed Columns

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RNA Cleanup Kit - Individual Packed Columns

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RNA Cleanup Kit - Individual Packed Columns

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EUR 107

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EUR 47.84
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P3040 1000/pack
EUR 47.84
Description: 0.2mL PCR tubes individual

miRNA Isolation Kit - NOT Individual Packed Columns

RMI004 each
EUR 3

miRNA Isolation Kit - NOT Individual Packed Columns

RMI050 each
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Presto FFPE RNA Mini Kit - Individual Packed Columns

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EUR 3

Presto FFPE RNA Mini Kit - Individual Packed Columns

RF050 each
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Presto FFPE RNA Mini Kit - Individual Packed Columns

RF100 each
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Total RNA Mini Kit (Plant) - Individual Packed Columns

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Total RNA Mini Kit (Plant) - Individual Packed Columns

RP100 each
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0.2 ml Individual PCR Tubes with attached Flat Caps

GP101 1,000 tubes
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0.5 ml Individual PCR Tubes with attached Flat Caps

GP103 1,000 tubes
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amfiRivert cDNA Systhesis Premix , Individual 0.2ml tube

R5001-048 48 rxns
EUR 448.8

amfiRivert cDNA Systhesis Premix , Individual 0.2ml tube

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Total RNA Mini Kit (Tissue) - Individual Packed Columns

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EUR 3

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Total RNA Mini Kit (Tissue) - Individual Packed Columns

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0.2 ml Individual PCR Tubes with attached Domed Caps

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0.5 ml Individual PCR Tubes with attached Domed Caps

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rSYNC™ RNA Isolation Kit - Individual Packed Columns

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RS050 each
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rSYNC™ RNA Isolation Kit - Individual Packed Columns

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rSYNC™ RNA Isolation Kit - Individual Packed Columns

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TriRNA Pure Kit w/DNase Set - Individual Packed Columns

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EUR 5

TriRNA Pure Kit w/DNase Set - Individual Packed Columns

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DB 6.2 Single block; 64 x 0.2ml individual PCR tubes - EACH

BLO6080 EACH
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Microtube PCR individual 0.2 mL green colour Treff - PK500

DD34041 PK500
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GENEzol™ TriRNA Pure Kit - NOT Individual Packed Columns

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Adaptor Cap Wide Mouth to 1/4" (5.8 mm) ID Hose for BioFactories, PP, Individual

740402 10 pcs/cs
EUR 7.15
Description: Adaptor Cap Wide Mouth to 1/4" (5.8 mm) ID Hose for BioFactories, PP, Individually Wrapped, Sterile, 1/pk, 10/cs

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RB Columns (Individual Packed Columns) WITHOUT Collection Tube

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PCR 8-Strips. 0.2ml with Attached Individual Flat Caps, 120/pk

P3010-A 120
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96 individual caps in sheet format blue TPE pierceable - PK50

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AmpliStar 0.2ml Individual PCR Tubes with Domed Caps - 1000 tubes

WB2-3510 each Ask for price

GENEzol™ TriRNA Bacteria Kit - NOT Individual Packed Columns

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EUR 3

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GENEzol™ TriRNA Bacteria Kit - NOT Individual Packed Columns

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GENEzol™ TriRNA Bacteria Kit - NOT Individual Packed Columns

GZB200 each
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MINI TUBE, INDIVIDUAL, POLYPROPYLENE, 0.65 ML, CLEAR, NONSTERILE, BULK

MTS-06-C 960/pk
EUR 211.2
Description: Deep Well/Assay Plates - Axygen; Assay/Other - Axygen

MINI TUBE, INDIVIDUAL, POLYPROPYLENE, 1.1 ML, CLEAR, NONSTERILE, BULK

MTS-11-C 960/pk
EUR 168
Description: Deep Well/Assay Plates - Axygen; Assay/Other - Axygen

DB 6.1 Single block; 10 x 8 individual PCR tube strips; 0.2ml - EACH

BLO6078 EACH
EUR 182.25

BD Iml in 1100 Serological pipette plugged Individual wrap - PK1000

357521 PK1000
EUR 241.65

Presto™ Mini RNA Yeast Advanced Kit - Individual Packed Columns

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EUR 6

Presto™ Mini RNA Yeast Advanced Kit - Individual Packed Columns

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EUR 130.5

Presto™ Mini RNA Yeast Advanced Kit - Individual Packed Columns

RBYA100 each
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Presto™ Mini RNA Yeast Advanced Kit - Individual Packed Columns

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Presto FFPE RNA Mini Kit w/DNase Set - Individual Packed Columns

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EUR 5

Presto FFPE RNA Mini Kit w/DNase Set - Individual Packed Columns

RFD050 each
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Presto FFPE RNA Mini Kit w/DNase Set - Individual Packed Columns

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EUR 193

Total RNA Mini Kit (Plant) w/DNase Set - Individual Packed Columns

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EUR 5

Total RNA Mini Kit (Plant) w/DNase Set - Individual Packed Columns

RPD050 each
EUR 107.8

Total RNA Mini Kit (Plant) w/DNase Set - Individual Packed Columns

RPD100 each
EUR 195.8

Total RNA Mini Kit (Plant) w/DNase Set - Individual Packed Columns

RPD300 each
EUR 520.2

Total RNA Mini Kit (Blood/Cultured Cell) - Individual Packed Columns

RB004 each
EUR 3

Total RNA Mini Kit (Blood/Cultured Cell) - Individual Packed Columns

RB050 each
EUR 82.3

Total RNA Mini Kit (Blood/Cultured Cell) - Individual Packed Columns

RB100 each
EUR 150.7

Total RNA Mini Kit (Blood/Cultured Cell) - Individual Packed Columns

RB300 each
EUR 396.5

Dispenser for an individual 12m long roll of ClearLine tape - EACH

DD188047 EACH
EUR 17.55

Total RNA Mini Kit (Tissue) w/DNase Set - Individual Packed Columns

RTD004 each
EUR 5

Total RNA Mini Kit (Tissue) w/DNase Set - Individual Packed Columns

RTD050 each
EUR 103.4

Total RNA Mini Kit (Tissue) w/DNase Set - Individual Packed Columns

RTD100 each
EUR 193

Total RNA Mini Kit (Tissue) w/DNase Set - Individual Packed Columns

RTD300 each
EUR 518

rSYNC™ RNA Isolation Kit w/DNase Set - Individual Packed Columns

RSD004 each
EUR 5

rSYNC™ RNA Isolation Kit w/DNase Set - Individual Packed Columns

RSD050 each
EUR 103.4

rSYNC™ RNA Isolation Kit w/DNase Set - Individual Packed Columns

RSD100 each
EUR 193

rSYNC™ RNA Isolation Kit w/DNase Set - Individual Packed Columns

RSD300 each
EUR 518

1.1ml Cluster Tubes, Individual Tube Format,Non-Sterile, 960/pk, 4800/cs

628111 4800 pcs/cs
EUR 52.47
Description: 1.1ml Cluster Tubes, Individual Tube Format,Non-Sterile, 960/pk, 4800/cs

MINI TUBE, INDIVIDUAL, POLYPROPYLENE, 0.65 ML, CLEAR, RACKED, STERILE, BULK

MTS-06-C-R-S 960/pk
EUR 517.2
Description: Deep Well/Assay Plates - Axygen; Assay/Other - Axygen

MINI TUBE, INDIVIDUAL, POLYPROPYLENE, 1.1 ML, CLEAR, RACKED, STERILE, BULK

MTS-11-C-R-S 960/pk
EUR 397.2
Description: Deep Well/Assay Plates - Axygen; Assay/Other - Axygen

Presto™ DNA/RNA/Protein Extraction Kit - Individual Packed Columns

DRP004 each
EUR 5

Presto™ DNA/RNA/Protein Extraction Kit - Individual Packed Columns

DRP050 each
EUR 104

Presto™ DNA/RNA/Protein Extraction Kit - Individual Packed Columns

DRP100 each
EUR 181.5

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