GMP-grade neural progenitor derivation and differentiation from clinical-grade human embryonic stem cells

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GMP-grade neural progenitor derivation and differentiation from clinical-grade human embryonic stem cells

Background: A serious problem for the medical use of human pluripotent stem cells is the event of secure, sturdy and managed differentiation protocols. Adaptation of analysis protocols utilizing reagents designated as research-only to these that are appropriate for medical use, also known as good manufacturing follow (GMP) reagents, is an important and laborious step within the translational pipeline. Nonetheless, revealed protocols to help this course of stay very restricted.

Strategies: We tailored research-grade protocols for the derivation and differentiation of long-term neuroepithelial stem cell progenitors (lt-NES) to GMP-grade reagents and elements appropriate for medical functions. We screened the robustness of the protocol with six clinical-grade hESC traces deposited within the UK Stem Cell Financial institution.

Outcomes: Right here, we current a brand new GMP-compliant protocol to derive lt-NES, that are multipotent, bankable and karyotypically steady. This protocol resulted in sturdy and reproducible differentiation of a number of clinical-grade embryonic stem cells from which we derived lt-NES. Moreover, GMP-derived lt-NES demonstrated a excessive neurogenic potential whereas retaining the flexibility to be redirected to a number of neuronal sub-types.

Conclusions: Total, we report the feasibility of derivation and differentiation of clinical-grade embryonic stem cell traces into lt-NES beneath GMP-compliant circumstances. Our protocols may very well be used as a versatile software to hurry up translation-to-clinic of pluripotent stem cells for a wide range of neurological therapies or regenerative medication research.

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G128 500 ul 10 mM each
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dNTP Mix
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dNTP Mix
K6011105-100 100 ul (10 mM each)
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dNTP Mix
K6011105-1000 1000 ul (10 mM each)
EUR 241.2
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dNTP Mix
K6011105-200 200 ul (10 mM each)
EUR 111.6
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dNTP Mix
K6011105-400 400 ul (10 mM each)
EUR 139.2
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Analyte Mix
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Analyte Mix
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Analyte Mix
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Analyte Mix
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Adrenal Dissociation System 4 (Heart, Adrenal chromaffin, Paraneurons), Mouse and Rat
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ExoStd? Lyophilized Exosome Standard (100 µg, COLO1 cell line, 4 vials)
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M1052-4
EUR 1096.8

ExoStd? Lyophilized Exosome Standard (100 µg, BLCL21 cell line, 4 vials)
M1053-4
EUR 1370.4

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