GMP-grade neural progenitor derivation and differentiation from clinical-grade human embryonic stem cells
Background: A serious problem for the medical use of human pluripotent stem cells is the event of secure, sturdy and managed differentiation protocols. Adaptation of analysis protocols utilizing reagents designated as research-only to these that are appropriate for medical use, also known as good manufacturing follow (GMP) reagents, is an important and laborious step within the translational pipeline. Nonetheless, revealed protocols to help this course of stay very restricted.
Strategies: We tailored research-grade protocols for the derivation and differentiation of long-term neuroepithelial stem cell progenitors (lt-NES) to GMP-grade reagents and elements appropriate for medical functions. We screened the robustness of the protocol with six clinical-grade hESC traces deposited within the UK Stem Cell Financial institution.
Outcomes: Right here, we current a brand new GMP-compliant protocol to derive lt-NES, that are multipotent, bankable and karyotypically steady. This protocol resulted in sturdy and reproducible differentiation of a number of clinical-grade embryonic stem cells from which we derived lt-NES. Moreover, GMP-derived lt-NES demonstrated a excessive neurogenic potential whereas retaining the flexibility to be redirected to a number of neuronal sub-types.
Conclusions: Total, we report the feasibility of derivation and differentiation of clinical-grade embryonic stem cell traces into lt-NES beneath GMP-compliant circumstances. Our protocols may very well be used as a versatile software to hurry up translation-to-clinic of pluripotent stem cells for a wide range of neurological therapies or regenerative medication research.
 Individual Reaction Mix 5 |
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| G065-5 | ABM | 200 reactions | EUR 167 |
 Individual Reaction Mix |
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| G065-X | ABM | 200 reactions | EUR 167 |
 Individual Reaction Mix 1 |
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| G065-1 | ABM | 200 reactions | EUR 167 |
 Individual Reaction Mix 10 |
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| G065-10 | ABM | 200 reactions | EUR 167 |
 Individual Reaction Mix 11 |
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| G065-11 | ABM | 200 reactions | EUR 167 |
 Individual Reaction Mix 12 |
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| G065-12 | ABM | 200 reactions | EUR 167 |
 Individual Reaction Mix 2 |
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| G065-2 | ABM | 200 reactions | EUR 167 |
 Individual Reaction Mix 3 |
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| G065-3 | ABM | 200 reactions | EUR 167 |
 Individual Reaction Mix 4 |
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| G065-4 | ABM | 200 reactions | EUR 167 |
 Individual Reaction Mix 6 |
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| G065-6 | ABM | 200 reactions | EUR 167 |
 Individual Reaction Mix 7 |
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| G065-7 | ABM | 200 reactions | EUR 167 |
 Individual Reaction Mix 8 |
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| G065-8 | ABM | 200 reactions | EUR 167 |
 Individual Reaction Mix 9 |
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| G065-9 | ABM | 200 reactions | EUR 167 |
 (Reaction Volume 30ul) (96 Tubes)) 2X PCR Pre-Mix (EF) (Reaction Volume 30ul) (96 Tubes) |
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| 9K-002-0001 | BBI Biotech | 96tubes, 96prep | EUR 311 |
|
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 (Reaction Volume 30ul) (576 Tubes)) 2X PCR Pre-Mix (EF) (Reaction Volume 30ul) (576 Tubes) |
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| 9K-002-0002 | BBI Biotech | 576tubes, 576prep | EUR 1159.25 |
|
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, for 50ul reaction) amfiEco PCR Master Mix,8-Strip RTU(2X), for 50ul reaction |
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| P0704-096 | GenDepot | 96 rxns | EUR 145 |
 Dream Media Nutrient Mix, 5 packets |
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| 180 | AthenaES | Pack 5 | EUR 77 |
) 2xEs Taq Master Mix (with Dyes) |
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| W0690-5 | 101Bio | NULL | EUR 0 |
 2X Reaction Buffer |
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| 1068-20 | Biovision | EUR 185 | |
2X Reaction Buffer |
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| 1068-80 | Biovision | EUR 294 | |
) 2×Taq PCR Master Mix(with dye) |
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| K1034-5 | ApexBio | 5×1 ml | EUR 119 |
) 1-Drop PCR Mix (squeeze bottle, no pipet) |
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| W2599-5 | 101Bio | NULL | EUR 0 |
-BSA Antigen (HM238) (Molar Reaction Ratio: 5:1)) Benzodiazepine (Oxazepam)-BSA Antigen (HM238) (Molar Reaction Ratio: 5:1) |
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| VAng-Cr3654-1mg | Creative Biolabs | 1 mg | EUR 394 |
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Description: Benzodiazepine (Oxazepam)-BSA Antigen, matched pairs: HM238(detection); Molar Reaction Ratio: 5:1 (Benzodiazepine:BSA); Used for Lateral Flow. |
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 RANGER Reaction Buffer, 5x |
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| BIO-37114 | Bioline | 3 x 1.25ml | Ask for price |
 MyFi Reaction Buffer, 5x |
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| BIO-37115 | Bioline | 3 x 1.25ml | Ask for price |
 amfiXpand 2x Reaction Buffer |
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| P0333-001 | GenDepot | 1ml | EUR 101 |
 amfiFusion 2x Reaction Buffer |
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| P0343-001 | GenDepot | 1ml | EUR 104 |
) Extra T7 gRNA PCR primer mix (5 uM) |
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| CAS510A-PR | SBI | 50 assays | EUR 153 |
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 Forward and Reverse UCR1 Primer Mix 5 ?M |
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| LV961A-UCR1 | SBI | 50 ul | EUR 165 |
|
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 dNTP Mix |
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| G010 | ABM | 250 ul 10 mM each | EUR 73 |
dNTP Mix |
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| G128 | ABM | 500 ul 10 mM each | EUR 82 |
dNTP Mix |
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| G129 | ABM | 1.0 ml 10 mM each | EUR 101 |
 NTP Mix |
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| G943 | ABM | 250 ul 25 mM each | EUR 78 |
NTP Mix |
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| G944 | ABM | 500 ul 25 mM each | EUR 97 |
NTP Mix |
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| G945 | ABM | 1.0 ml 25 mM each | EUR 130 |
dNTP Mix |
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| K6011105-100 | Biochain | 100 ul (10 mM each) | EUR 86 |
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Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
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dNTP Mix |
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| K6011105-1000 | Biochain | 1000 ul (10 mM each) | EUR 201 |
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Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
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dNTP Mix |
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| K6011105-200 | Biochain | 200 ul (10 mM each) | EUR 93 |
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Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
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dNTP Mix |
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| K6011105-400 | Biochain | 400 ul (10 mM each) | EUR 116 |
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Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
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 PCR Mix |
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| L5051100 | Biochain | 2.5 ml | EUR 107 |
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Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
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) IL-8 (-5 to +5) |
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| 5-01377 | CHI Scientific | 4 x 5mg | Ask for price |
) Ultra SYBR Green qPCR Master Mix (2X, with ROX I) |
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| W2601-5 | 101Bio | NULL | EUR 0 |
 Hi-Fi Reaction Buffer, 5x |
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| BIO-37110 | Bioline | 2 x 1.5ml | Ask for price |
 MyTaq reaction Buffer Colourless, 5x |
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| BIO-37111 | Bioline | 4 x 1ml | Ask for price |
 MyTaq Reaction Buffer Red, 5x |
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| BIO-37112 | Bioline | 4 x 1ml | Ask for price |
) amfiEco Taq Reaction Buffer(2X) |
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| P0703-001 | GenDepot | 1ml | EUR 84 |
amfiEco Taq Reaction Buffer(2X) |
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| P0703-005 | GenDepot | 5ml | EUR 178 |
 Kinase Reaction Buffer Concentrate, 60ML |
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| X104-60ML | Arbor Assays | 60ML | EUR 122 |
 2',3',5'-triacetyl-5-Azacytidine |
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| B2822-5 | Biovision | EUR 153 | |
2',3',5'-triacetyl-5-Azacytidine |
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| C4119-5 | ApexBio | 5 mg | EUR 118 |
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Description: 2',3',5'-triacetyl-5-Azacytidine (TAC) is the lead prodrug form of 5-azacytidine which may be rapidly absorbed after oral administration [1]. 5-Azacytidine is an inhibitor of DNA methyltransferase [2]. |
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, 20 ul/rxn) 2x GoldStar Best PCR Master Mix (with Dyes), 20 ul/rxn |
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| W0655-5 | 101Bio | NULL | EUR 0 |
 Aftin-5 |
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| 2575-5 | Biovision | EUR 457 | |
 CHD-5 |
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| 2693-5 | Biovision | EUR 153 | |
 Necrostatin-5 |
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| 1865-5 | Biovision | EUR 126 | |
 Histatin 5 |
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| 5-01307 | CHI Scientific | 4 x 1mg | Ask for price |
 GNF-5 |
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| B1329-5 | Biovision | EUR 142 | |
 5-ROX |
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| B8212-5 | ApexBio | 5 mg | EUR 195 |
 5-Iodotubercidin |
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| A3125-5 | ApexBio | 5 mg | EUR 168 |
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Description: 5-Iodotubercidin (Itu) is a purine derivative and hence an inhibitor of adenosine kinase with an IC50 value of 26 nM [1]. Adenosine kinase is important in regulating the intracellular and extracellular concentrations of adenosine and hence diverse physiological actions of adenosine [2]. |
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) Thrombin Receptor Activator for Peptide 5 (TRAP-5) |
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| A1036-5 | ApexBio | 5 mg | EUR 102 |
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Description: Thrombin Receptor Activator for Peptide 5 (TRAP-5),(C30H50N8O7), a peptide with the sequence NH2-Ser-Phe-Leu-Leu-Arg-COOH, MW= 634.76. coagulation factor II (thrombin) receptor is a G protein-coupled receptor involved in the regulation of thrombotic response. |
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 2',5'-Dideoxyadenosine |
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| 2770-5 | Biovision | EUR 240 | |
 TRAP-5 amide |
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| 5-02019 | CHI Scientific | 4 x 5mg | Ask for price |
 [Cit5]-TRAP-5 |
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| 5-00062 | CHI Scientific | 4 x 5mg | Ask for price |
 A-VI-5 |
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| 5-00524 | CHI Scientific | 4 x 5mg | Ask for price |
) ACTH(5-10) |
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| 5-00605 | CHI Scientific | 4 x 5mg | Ask for price |
) Angiotensin (1-5) |
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| 5-00676 | CHI Scientific | 4 x 5mg | Ask for price |
) Bradykinin (1-5) |
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| 5-00852 | CHI Scientific | 4 x 5mg | Ask for price |
 W-5 hydrochloride |
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| B6277-5 | ApexBio | 5 mg | EUR 116 |
 5-Nonyloxytryptamine oxalate |
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| B6496-5 | ApexBio | 5 mg | EUR 154 |
 Teicoplanin A2-5 |
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| C4938-5 | ApexBio | 5 mg | EUR 1157 |
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Description: Teicoplanin A2-5, derived from Actinoplanes teichomyceticus, is a glycopeptide antibiotic which produces a broad spectrum of antibiotic activity against gram-positive bacteria. |
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 5-fluoro 203 |
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| C4114-5 | ApexBio | 5 mg | EUR 154 |
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Description: 5-fluoro 203 (5F-203) is an antitumor agent and cytotoxic compound that acts as a potent AhR agonist [1][2][3]. The aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor involved in regulating xenobiotic-metabolizing enzymes such as cytochrome P450. |
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 5'-deoxy Thymidine |
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| C4692-5 | ApexBio | 5 mg | EUR 180 |
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Description: 5'-Deoxy Thymidine has been used as a research tool for antiviral and anticancer studies. 5'-Deoxy Thymidine can be used to study the entry mechanism into human erythrocytes in comparison with other deoxythymidines. |
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 Glucose Nutrient Mix |
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| 109 | AthenaES | 150 g | EUR 67 |
 dNTP/dUTP Mix |
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| DB2236 | BBI Biotech | 1ml | EUR 58.7 |
|
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 ACCUZYME Mix, 2x |
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| BIO-25028 | Bioline | 500 Reactions | Ask for price |
 MyTaq Mix, 2x |
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| BIO-25041 | Bioline | 200 Reactions | Ask for price |
MyTaq Mix, 2x |
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| BIO-25041/S | Bioline | Sample | Ask for price |
MyTaq Mix, 2x |
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| BIO-25042 | Bioline | 1000 Reactions | Ask for price |
 MyFi Mix, 2x |
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| BIO-25049 | Bioline | 100 Reactions | Ask for price |
MyFi Mix, 2x |
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| BIO-25049/S | Bioline | Sample | Ask for price |
MyFi Mix, 2x |
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| BIO-25050 | Bioline | 500 Reactions | Ask for price |
 Ranger Mix, 2x |
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| BIO-25051/S | Bioline | Sample | Ask for price |
Ranger Mix, 2x |
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| BIO-25052 | Bioline | 500 Reactions | Ask for price |
 Green Taq Mix |
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| P131-01 | Vazyme | 5 ml | EUR 119 |
Green Taq Mix |
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| P131-02 | Vazyme | 15 ml | EUR 150 |
Green Taq Mix |
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| P131-03 | Vazyme | 50 ml | EUR 248 |
 Jade? Master Mix |
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| M1105-500 | Biovision | EUR 441 | |
 Jade? Mix-iCycler |
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| M1106-500 | Biovision | EUR 441 | |
 Jade? Mix-ROX |
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| M1108-500 | Biovision | EUR 441 | |
 Jade Express? Mix |
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| M1109-500 | Biovision | EUR 441 | |
 Jade Smart? Mix |
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| M1113-500 | Biovision | EUR 441 | |
 Jade miRNA? Mix |
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| M1117-500 | Biovision | EUR 441 | |
 Taqman Master Mix |
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| M1121-500 | Biovision | EUR 441 | |
 Ready? PCR Mix |
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| M1127-1000 | Biovision | EUR 512 | |
Ready? PCR Mix |
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| M1127-200 | Biovision | EUR 191 | |
 Rigor? PCR Mix |
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| M1132-200 | Biovision | EUR 321 | |
 Breeze? PCR Mix |
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| M1134-200 | Biovision | EUR 267 | |
 Distant? PCR Mix |
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| M1136-200 | Biovision | EUR 294 | |
 Advance? PCR Mix |
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| M1139-200 | Biovision | EUR 376 | |
 Aflatoxin MIX Standard |
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| SD009 | Unibiotest | 0.5ug/mL | EUR 607 |
 INDIVIDUAL ADAPTERS FOR 0.5ML TUBES,1/6 |
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| 480134 | CORNING | 6/pk | EUR 63 |
|
Description: Lab Equipment; General Purpose Centrifuges (affliated brand) |
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 INDIVIDUAL ADAPTERS FOR 0.2ML TUBES,1/6 |
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| 480135 | CORNING | 6/pk | EUR 63 |
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Description: Lab Equipment; General Purpose Centrifuges (affliated brand) |
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 amfiRivert cDNA Systhesis Premix , Individual 0.2ml tube |
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| R5001-048 | GenDepot | 48 rxns | EUR 374 |
amfiRivert cDNA Systhesis Premix , Individual 0.2ml tube |
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| R5001-096 | GenDepot | 96 rxns | EUR 874 |
) amfiRivert Reverse Transcriptase Reaction Buffer(5X) |
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| A1203-001 | GenDepot | 1ml | EUR 88 |
 ELISA Kit) 5-Formylcytosine (5-fC) ELISA Kit |
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| MET-5102-5 | Cell Biolabs | 5 x 96 assays | EUR 2283 |
 ELISA Kit) 5-Carboxylcytosine (5-caC) ELISA Kit |
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| MET-5103-5 | Cell Biolabs | 5 x 96 assays | EUR 2283 |
 Necrosis Inhibitor, Necrox-5 |
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| 2229-5 | Biovision | EUR 615 | |
, porcine) Secretin (5 - 27), porcine |
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| 5-01917 | CHI Scientific | 4 x 1mg | Ask for price |
) SH2 Domain Ligand (5) |
|||
| 5-01929 | CHI Scientific | 4 x 5mg | Ask for price |
 Boc-5-Ava-OH |
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| 5-04607 | CHI Scientific | 25g | Ask for price |
Boc-5-Ava-OH |
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| 5-04608 | CHI Scientific | 100g | Ask for price |
 Fmoc-5-Ava-OH |
|||
| 5-04609 | CHI Scientific | 5g | Ask for price |
Fmoc-5-Ava-OH |
|||
| 5-04610 | CHI Scientific | 25g | Ask for price |
) Dok- 5 (263 - 275) |
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| 5-01048 | CHI Scientific | 4 x 5mg | Ask for price |
 5-Nitroso-8-quinolinol |
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| B2825-5 | Biovision | EUR 109 | |
 AICA riboside, 5'-phosphate |
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| 9416-5 | Biovision | EUR 153 | |
 5-trans U-46619 |
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| C4261-5 | ApexBio | 5 mg | EUR 538 |
|
Description: 5-trans U-46619 is a minor impurity (2-5%) which variably exists in most commercial preparations of U-46619 [1]. U-46619 is a selective thromboxane A2 agonist involved in inducing platelet aggregation and vascular smooth muscle contraction [2]. |
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 2',5'-dideoxy Adenosine |
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| C3993-5 | ApexBio | 5 mg | EUR 189 |
|
Description: 2',5'-dideoxy Adenosine, a nucleoside analog, is one of the first identified cell-permeable, P-site inhibitors of adenylate cyclase [1]. Adenylyl cyclase is an enzyme with key regulatory roles in almost all cells. |
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carboxamidoadenosine) 5'-(N-Cyclopropyl)carboxamidoadenosine |
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| C4645-5 | ApexBio | 5 mg | EUR 128 |
 FAM azide, 5- isomer |
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| A8117-5 | ApexBio | 5 mg | EUR 166 |
|
Description: FAM azide for Click chemistry labeling. FAM remains one of the most popular fluorescent lablels for various application. Most instruments capable of fluorescence detection, ranging from plate readers to fluorescence microscopes, are able to work in FAM channel. |
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 ROX azide, 5- isomer |
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| A8126-5 | ApexBio | 5 mg | EUR 583 |
|
Description: ROX (rhodamine-X) azide, solid material, and 10 mM solution in DMSO, labeling reagent for Click Chemistry. ROX is a red-emitting rhodamine dye possessing high brightness and fluorescence quantum yield.Pure 5-isomer. |
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 TAMRA azide, 5- isomer |
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| A8129-5 | ApexBio | 5 mg | EUR 583 |
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Description: TAMRA (TMR, tetramethylrhodamine) azide, solid compound, and 10 mM solution in DMSO, labeling reagent for Click Chemistry. Pure 5-isomer.TAMRA is often used as FRET acceptor for FAM fluorophore. |
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 labeling) Mix-n-Stain CF350 Antibody Labeling Kit, 1x(5-20ug) labeling |
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| 92270 | Biotium | 1KIT | EUR 161 |
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Description: Minimum order quantity: 1 unit of 1KIT |
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 labeling) Mix-n-Stain CF405S Antibody Labeling Kit, 1x(5-20ug) labeling |
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| 92271 | Biotium | 1KIT | EUR 161 |
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Description: Minimum order quantity: 1 unit of 1KIT |
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 labeling) Mix-n-Stain CF405M Antibody Labeling Kit, 1x(5-20ug) labeling |
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| 92272 | Biotium | 1KIT | EUR 161 |
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Description: Minimum order quantity: 1 unit of 1KIT |
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 labeling) Mix-n-Stain CF488A Antibody Labeling Kit, 1x(5-20ug) labeling |
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| 92273 | Biotium | 1KIT | EUR 161 |
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Description: Minimum order quantity: 1 unit of 1KIT |
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 labeling) Mix-n-Stain CF555 Antibody Labeling Kit, 1x(5-20ug) labeling |
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| 92274 | Biotium | 1KIT | EUR 161 |
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Description: Minimum order quantity: 1 unit of 1KIT |
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Dynamic Laser Speckle Imaging meets Machine Studying to allow Fast Antibacterial Susceptibility Testing (DyRAST)
Fast antibacterial susceptibility testing (RAST) strategies which measure change of a bacterial phenotype in response to a given therapy are of great significance in healthcare, as they’ll help care-givers within the well timed administration of appropriate remedies. Numerous RAST strategies have been reported for monitoring bacterial phenotypes, together with dimension, form, movement, and redox state. Nonetheless, they nonetheless require cumbersome and costly devices – which hinders their software in resource-limited environments – and/or make the most of labeling reagents which may intervene with antibiotics and add to price.
Moreover, the present RAST strategies don’t deal with the potential gradual adaptation of micro organism to antibiotics, which may result in a false prognosis. On this work, we current a RAST strategy by leveraging machine studying to research time-resolved dynamic laser speckle imaging (DLSI) outcomes. DLSI captures change of bacterial movement/division in response to antibiotic remedies.
Our technique precisely predicts the minimal inhibitory focus (MIC) of ampicillin and gentamicin for a mannequin pressure of Escherichia coli (E. coli Ok-12) in 60 minutes, in comparison with 6 hours utilizing the at the moment FDA-approved phenotype-based RAST method. Along with ampicillin (a β-lactam) and gentamicin (an aminoglycoside), we studied the impact of ceftriaxone (a third-generation medical cephalosporin) on E. coli Ok-12. The machine studying algorithm was skilled and validated utilizing the in a single day outcomes of a gold customary AST technique enabling prediction of MIC with a equally excessive accuracy, but considerably sooner.
Polymer-Supported Phosphoric, Phosphonic and Phosphinic Acids-From Synthesis to Properties and Purposes in Separation Processes
Environment friendly separation applied sciences are essential to the atmosphere and world financial system. The problem posed to scientists is how one can engineer selectivity in the direction of a focused substrate, particularly from multicomponent options. Polymer-supported reagents have gained plenty of consideration on this context, as they eradicate plenty of inconveniences regarding broadly used solvent extraction strategies.
However, the selection of an applicable ligand for immobilization could also be derived from the habits of soluble compounds beneath solvent extraction circumstances. Organophosphorus compounds play a big function in separation science and expertise. The options they possess, equivalent to variable oxidation states, multivalence, asymmetry and metal-binding properties, spotlight their standing as a novel and versatile class of compounds, able to selective separations continuing by means of completely different mechanisms.
This evaluate supplies an in depth survey of polymers containing phosphoric, phosphonic and phosphinic acid functionalities within the aspect chain and covers foremost advances within the preparation and software of those supplies in separation science, together with essentially the most related synthesis routes (Arbuzov, Perkow, Mannich, Kabachnik-Fields reactions, and many others.), in addition to the primary levels within the improvement of organophosphorus resins and a very powerful achievements within the subject.
Individual Reaction Mix 12 |
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| G065-12 | ABM | 200 reactions | EUR 167 |
Individual Reaction Mix 2 |
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| G065-2 | ABM | 200 reactions | EUR 167 |
Individual Reaction Mix 3 |
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| G065-3 | ABM | 200 reactions | EUR 167 |
Individual Reaction Mix 5 |
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| G065-5 | ABM | 200 reactions | EUR 167 |
Individual Reaction Mix 6 |
|||
| G065-6 | ABM | 200 reactions | EUR 167 |
Individual Reaction Mix 7 |
|||
| G065-7 | ABM | 200 reactions | EUR 167 |
Individual Reaction Mix 8 |
|||
| G065-8 | ABM | 200 reactions | EUR 167 |
Individual Reaction Mix 9 |
|||
| G065-9 | ABM | 200 reactions | EUR 167 |
2X PCR Pre-Mix (EF) (Reaction Volume 30ul) (96 Tubes) |
|||
| 9K-002-0001 | BBI Biotech | 96tubes, 96prep | EUR 311 |
|
|||
2X PCR Pre-Mix (EF) (Reaction Volume 30ul) (576 Tubes) |
|||
| 9K-002-0002 | BBI Biotech | 576tubes, 576prep | EUR 1159.25 |
|
|||
amfiEco PCR Master Mix,8-Strip RTU(2X), for 50ul reaction |
|||
| P0704-096 | GenDepot | 96 rxns | EUR 145 |
) 40mM dNTP Mix (10mM/80µm each) |
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| PR3040-M-4 | BenchMark | 1 PC | EUR 159.11 |
|
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2X Reaction Buffer |
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| 1068-20 | Biovision | EUR 185 | |
2X Reaction Buffer |
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| 1068-80 | Biovision | EUR 294 | |
 IL-4 Interleukin 4 Human Recombinant Protein, Yeast |
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| PROTP05112-4 | BosterBio | Regular: 10ug | EUR 317 |
|
Description: Interleukin-4 Human Recombinant produced in yeast is a single, glycosylated polypeptide chain containing 129 amino acids.;The IL-4 is purified by proprietary chromatographic techniques. |
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RANGER Reaction Buffer, 5x |
|||
| BIO-37114 | Bioline | 3 x 1.25ml | Ask for price |
MyFi Reaction Buffer, 5x |
|||
| BIO-37115 | Bioline | 3 x 1.25ml | Ask for price |
amfiXpand 2x Reaction Buffer |
|||
| P0333-001 | GenDepot | 1ml | EUR 101 |
amfiFusion 2x Reaction Buffer |
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| P0343-001 | GenDepot | 1ml | EUR 104 |
 pLenti-CLDN1 shRNA-4 Plasmid |
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| PVTBAV04867-4 | Lifescience Market | 2 ug | EUR 356 |
 Feline IL-4 Recombinant Protein |
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| R00230-4 | BosterBio | 5ug/vial | EUR 259 |
|
Description: IL-4 has many biological roles, including the stimulation of activated B-cell and T-cell proliferation, and the differentiation of CD4+ T-cells into Th2 cells. It is a key regulator in humoral and adaptive immunity. Feline IL-4 Recombinant Protein is purified interleukin-4 produced in yeast. |
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dNTP Mix |
|||
| G010 | ABM | 250 ul 10 mM each | EUR 73 |
dNTP Mix |
|||
| G128 | ABM | 500 ul 10 mM each | EUR 82 |
dNTP Mix |
|||
| G129 | ABM | 1.0 ml 10 mM each | EUR 101 |
NTP Mix |
|||
| G943 | ABM | 250 ul 25 mM each | EUR 78 |
NTP Mix |
|||
| G944 | ABM | 500 ul 25 mM each | EUR 97 |
NTP Mix |
|||
| G945 | ABM | 1.0 ml 25 mM each | EUR 130 |
dNTP Mix |
|||
| K6011105-100 | Biochain | 100 ul (10 mM each) | EUR 86 |
|
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
|||
dNTP Mix |
|||
| K6011105-1000 | Biochain | 1000 ul (10 mM each) | EUR 201 |
|
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
|||
dNTP Mix |
|||
| K6011105-200 | Biochain | 200 ul (10 mM each) | EUR 93 |
|
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
|||
dNTP Mix |
|||
| K6011105-400 | Biochain | 400 ul (10 mM each) | EUR 116 |
|
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
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PCR Mix |
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| L5051100 | Biochain | 2.5 ml | EUR 107 |
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Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
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 DIGITAL SHAKER FOR 4 MICROPLATES, 120V |
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| 6780-4 | CORNING | 1/pk | EUR 798 |
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Description: Lab Equipment; Shakers & Mixers |
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 Mouse FibrOut 4, for brain, neural |
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| 4-20507 | CHI Scientific | 1 ml | Ask for price |
Mouse FibrOut 4, for brain, neural |
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| 4-20508 | CHI Scientific | 5 x 1 ml | Ask for price |
 Rat FibrOut 4, for brain, neural |
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| 4-20533 | CHI Scientific | 1 ml | Ask for price |
Rat FibrOut 4, for brain, neural |
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| 4-20534 | CHI Scientific | 5 x 1 ml | Ask for price |
 Human FibrOut 4, for brain, neural |
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| 4-21552 | CHI Scientific | 1 ml | Ask for price |
Human FibrOut 4, for brain, neural |
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| 4-21553 | CHI Scientific | 5 x 1 ml | Ask for price |
 Recombinant Human 4-1BB Receptor Protein |
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| PROTQ07011-4 | BosterBio | 20ug | EUR 317 |
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Description: 4-1BB Receptor, a member of the TNF superfamily of receptors, is mainly expressed on the surface of a variety of T cells, but also found in B cells, monocytes, and various transformed cell lines. 4-1BB Receptor binds to 4-1BBL to provide a co-stimulatory signal for T lymphocytes. Signaling by 4-1BB Receptor has been implicated in the antigen-presentation process and generation of cytotoxic T cells. The human 4-1BB Receptor gene codes for a 255 amino acid type I transmembrane protein containing a 17 amino acid N-terminal signal sequence, a 169 amino acid extracellular domain, a 27 amino acid transmembrane domain and a 42 amino acid cytoplasmic domain. Recombinant human soluble 4-1BB Receptor is a 167 amino acid polypeptide (17.7 kDa), which contains the cysteine rich TNFR-like extracellular domain of 4-1BB Receptor. |
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 Protein) Recombinant Human PF-4 (CXCL4) Protein |
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| PROTP02776-4 | BosterBio | 20ug | EUR 317 |
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Description: PF-4 is a CXC chemokine that is expressed in megakaryocytes and stored in the α-granules of platelets. PF-4 is chemotactic towards neutrophils and monocytes and has been shown to inhibit angiogenesis. Recombinant human PF-4 is a 7.8 kDa protein containing 70 amino acid residues, including the four highly conserved residues present in CXC chemokines. |
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, Adult Mouse) Bone Dissociation System 4 (Osteoblasts), Adult Mouse |
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| 4-20214 | CHI Scientific | ea | Ask for price |
, Rat) Liver Dissociation System 4 (Hepatocytes, rat), Rat |
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| 4-20304 | CHI Scientific | ea | Ask for price |
, Adult mouse) Pituitary Dissociation System 4 (Pituitary), Adult mouse |
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| 4-20404 | CHI Scientific | ea | Ask for price |
, Adult rat) Thymus Dissociation System 4 (Epithelial), Adult rat |
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| 4-20444 | CHI Scientific | ea | Ask for price |
Hi-Fi Reaction Buffer, 5x |
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| BIO-37110 | Bioline | 2 x 1.5ml | Ask for price |
MyTaq reaction Buffer Colourless, 5x |
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| BIO-37111 | Bioline | 4 x 1ml | Ask for price |
MyTaq Reaction Buffer Red, 5x |
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| BIO-37112 | Bioline | 4 x 1ml | Ask for price |
amfiEco Taq Reaction Buffer(2X) |
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| P0703-001 | GenDepot | 1ml | EUR 84 |
amfiEco Taq Reaction Buffer(2X) |
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| P0703-005 | GenDepot | 5ml | EUR 178 |
Kinase Reaction Buffer Concentrate, 60ML |
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| X104-60ML | Arbor Assays | 60ML | EUR 122 |
 DIGITAL SHAKER FOR 4 MICROPLATES, 230V-EU PLUG |
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| 6781-4 | CORNING | 1/pk | EUR 798 |
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Description: Lab Equipment; Shakers & Mixers |
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 DIGITAL SHAKER FOR 4 MICROPLATES, 230V-UK PLUG |
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| 6782-4 | CORNING | 1/pk | EUR 798 |
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Description: Lab Equipment; Shakers & Mixers |
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, Mouse and Rat) Cartilage Dissociation System 4 (Chondrocytes), Mouse and Rat |
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| 4-20234 | CHI Scientific | ea | Ask for price |
, Mouse) Mammary Dissociation System 4 (Epithelial, Swiss 3T3), Mouse |
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| 4-20324 | CHI Scientific | ea | Ask for price |
, Mouse and Rat) Pancreas Dissociation System 4 (Islets), Mouse and Rat |
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| 4-20374 | CHI Scientific | ea | Ask for price |
, Mouse and Rat) Skin Dissociation System 4 (Keratinocytes), Mouse and Rat |
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| 4-20434 | CHI Scientific | ea | Ask for price |
, Mouse and Rat) Adipose/Fat Dissociation System 4 (Predipocytes), Mouse and Rat |
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| 4-20194 | CHI Scientific | ea | Ask for price |
, Mouse and Rat) Brain Dissociation System 4 (Endothelial, Microvessels), Mouse and Rat |
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| 4-20224 | CHI Scientific | ea | Ask for price |
, Mouse and Rat) Endothelial Dissociation System 4 (Endothelial,Cerebral), Mouse and Rat |
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| 4-20244 | CHI Scientific | ea | Ask for price |
, Mouse and Rat) Heart Dissociation System 4 (Myocytes,Ventricles), Mouse and Rat |
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| 4-20274 | CHI Scientific | ea | Ask for price |
, Mouse and Rat) Muscle Dissociation System 4 (Smooth muscle), Mouse and Rat |
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| 4-20334 | CHI Scientific | ea | Ask for price |
, Mouse and Rat) Parotid Dissociation System 4 (Parotid acinar), Mouse and Rat |
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| 4-20394 | CHI Scientific | ea | Ask for price |
Glucose Nutrient Mix |
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| 109 | AthenaES | 150 g | EUR 67 |
dNTP/dUTP Mix |
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| DB2236 | BBI Biotech | 1ml | EUR 58.7 |
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ACCUZYME Mix, 2x |
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| BIO-25028 | Bioline | 500 Reactions | Ask for price |
MyTaq Mix, 2x |
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| BIO-25041 | Bioline | 200 Reactions | Ask for price |
MyTaq Mix, 2x |
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| BIO-25041/S | Bioline | Sample | Ask for price |
MyTaq Mix, 2x |
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| BIO-25042 | Bioline | 1000 Reactions | Ask for price |
MyFi Mix, 2x |
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| BIO-25049 | Bioline | 100 Reactions | Ask for price |
MyFi Mix, 2x |
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| BIO-25049/S | Bioline | Sample | Ask for price |
MyFi Mix, 2x |
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| BIO-25050 | Bioline | 500 Reactions | Ask for price |
Ranger Mix, 2x |
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| BIO-25051/S | Bioline | Sample | Ask for price |
Ranger Mix, 2x |
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| BIO-25052 | Bioline | 500 Reactions | Ask for price |
Green Taq Mix |
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| P131-01 | Vazyme | 5 ml | EUR 119 |
Green Taq Mix |
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| P131-02 | Vazyme | 15 ml | EUR 150 |
Green Taq Mix |
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| P131-03 | Vazyme | 50 ml | EUR 248 |
Jade? Master Mix |
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| M1105-500 | Biovision | EUR 441 | |
Jade? Mix-iCycler |
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| M1106-500 | Biovision | EUR 441 | |
Jade? Mix-ROX |
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| M1108-500 | Biovision | EUR 441 | |
Jade Express? Mix |
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| M1109-500 | Biovision | EUR 441 | |
Jade Smart? Mix |
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| M1113-500 | Biovision | EUR 441 | |
Jade miRNA? Mix |
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| M1117-500 | Biovision | EUR 441 | |
Taqman Master Mix |
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| M1121-500 | Biovision | EUR 441 | |
Ready? PCR Mix |
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| M1127-1000 | Biovision | EUR 512 | |
Ready? PCR Mix |
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| M1127-200 | Biovision | EUR 191 | |
Rigor? PCR Mix |
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| M1132-200 | Biovision | EUR 321 | |
Breeze? PCR Mix |
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| M1134-200 | Biovision | EUR 267 | |
Distant? PCR Mix |
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| M1136-200 | Biovision | EUR 294 | |
Advance? PCR Mix |
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| M1139-200 | Biovision | EUR 376 | |
Aflatoxin MIX Standard |
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| SD009 | Unibiotest | 0.5ug/mL | EUR 607 |
, Mouse and Rat) Epithelial Dissociation System 4 (Epithelial,Submandibular salivary), Mouse and Rat |
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| 4-20254 | CHI Scientific | ea | Ask for price |
, Mouse and Rat) Heart Dissociation System 4 (Retinal pigment epithelial), Mouse and Rat |
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| 4-20294 | CHI Scientific | ea | Ask for price |
, Mouse and Rat) Lung Dissociation System 4 (Alveolar type II), Mouse and Rat |
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| 4-20314 | CHI Scientific | ea | Ask for price |
, Mouse and Rat) Reproductive Tissue Dissociation System 4 (Epithelial, vagina), Mouse and Rat |
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| 4-20414 | CHI Scientific | ea | Ask for price |
) ExoStd? Lyophilized Exosome Standard (30 µg, Human Plasma, 4 vials) |
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| M1040-4 | Biovision | EUR 909 | |
) ExoStd? Lyophilized Exosome Standard (100 µg, Human Plasma, 4 vials) |
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| M1041-4 | Biovision | EUR 1137 | |
) ExoStd? Lyophilized Exosome Standard (30 µg, Human Serum, 4 vials) |
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| M1042-4 | Biovision | EUR 909 | |
) ExoStd? Lyophilized Exosome Standard (100 µg, Human Serum, 4 vials) |
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| M1043-4 | Biovision | EUR 1132 | |
) ExoStd? Lyophilized Exosome Standard (30 µg, Human Urine, 4 vials) |
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| M1044-4 | Biovision | EUR 909 | |
) ExoStd? Lyophilized Exosome Standard (100 µg, Human Urine, 4 vials) |
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| M1045-4 | Biovision | EUR 1137 | |
) ExoStd? Lyophilized Exosome Standard (30 µg, Human Saliva, 4 vials) |
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| M1046-4 | Biovision | EUR 914 | |
) ExoStd? Lyophilized Exosome Standard (100 µg, Human Saliva, 4 vials) |
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| M1047-4 | Biovision | EUR 1137 | |
) ExoStd? Lyophilized Exosome Standard (30 µg, U87 MG, 4 vials) |
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| M1054-4 | Biovision | EUR 914 | |
) ExoStd? Lyophilized Exosome Standard (100 µg, U87 MG, 4 vials) |
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| M1055-4 | Biovision | EUR 1142 | |
INDIVIDUAL ADAPTERS FOR 0.5ML TUBES,1/6 |
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| 480134 | CORNING | 6/pk | EUR 63 |
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Description: Lab Equipment; General Purpose Centrifuges (affliated brand) |
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INDIVIDUAL ADAPTERS FOR 0.2ML TUBES,1/6 |
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| 480135 | CORNING | 6/pk | EUR 63 |
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Description: Lab Equipment; General Purpose Centrifuges (affliated brand) |
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amfiRivert cDNA Systhesis Premix , Individual 0.2ml tube |
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| R5001-048 | GenDepot | 48 rxns | EUR 374 |
amfiRivert cDNA Systhesis Premix , Individual 0.2ml tube |
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| R5001-096 | GenDepot | 96 rxns | EUR 874 |
amfiRivert Reverse Transcriptase Reaction Buffer(5X) |
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| A1203-001 | GenDepot | 1ml | EUR 88 |
, Mouse and Rat) Adrenal Dissociation System 4 (Heart, Adrenal chromaffin, Paraneurons), Mouse and Rat |
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| 4-20204 | CHI Scientific | ea | Ask for price |
, Mouse and Rat) Kidney Dissociation System 4 (Inner medullary duct,Papillae), Mouse and Rat |
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| 4-29294 | CHI Scientific | ea | Ask for price |
) ExoStd? Lyophilized Exosome Standard (30 µg, COLO1 cell line, 4 vials) |
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| M1048-4 | Biovision | EUR 914 | |
) ExoStd? Lyophilized Exosome Standard (100 µg, COLO1 cell line, 4 vials) |
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| M1049-4 | Biovision | EUR 1142 | |
) ExoStd? Lyophilized Exosome Standard (30 µg, MM1 cell line, 4 vials) |
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| M1050-4 | Biovision | EUR 914 | |
) ExoStd? Lyophilized Exosome Standard (100 µg, MM1 cell line, 4 vials) |
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| M1051-4 | Biovision | EUR 1142 | |
) ExoStd? Lyophilized Exosome Standard (30 µg, BLCL21 cell line, 4 vials) |
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| M1052-4 | Biovision | EUR 914 | |