GMP-grade neural progenitor derivation and differentiation from clinical-grade human embryonic stem cells

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GMP-grade neural progenitor derivation and differentiation from clinical-grade human embryonic stem cells

Background: A serious problem for the medical use of human pluripotent stem cells is the event of secure, sturdy and managed differentiation protocols. Adaptation of analysis protocols utilizing reagents designated as research-only to these that are appropriate for medical use, also known as good manufacturing follow (GMP) reagents, is an important and laborious step within the translational pipeline. Nonetheless, revealed protocols to help this course of stay very restricted.

Strategies: We tailored research-grade protocols for the derivation and differentiation of long-term neuroepithelial stem cell progenitors (lt-NES) to GMP-grade reagents and elements appropriate for medical functions. We screened the robustness of the protocol with six clinical-grade hESC traces deposited within the UK Stem Cell Financial institution.

Outcomes: Right here, we current a brand new GMP-compliant protocol to derive lt-NES, that are multipotent, bankable and karyotypically steady. This protocol resulted in sturdy and reproducible differentiation of a number of clinical-grade embryonic stem cells from which we derived lt-NES. Moreover, GMP-derived lt-NES demonstrated a excessive neurogenic potential whereas retaining the flexibility to be redirected to a number of neuronal sub-types.

Conclusions: Total, we report the feasibility of derivation and differentiation of clinical-grade embryonic stem cell traces into lt-NES beneath GMP-compliant circumstances. Our protocols may very well be used as a versatile software to hurry up translation-to-clinic of pluripotent stem cells for a wide range of neurological therapies or regenerative medication research.

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Description: IDO1 Reaction Solution is an optimized, proprietary formulation containing all the necessary reaction components for IDO1-catalyzed conversion of L-tryptophan (L-Trp) to kynurenine (Kyn) in in vitro enzyme activity assays. This product is intended to be used with the BPS IDO1 Inhibitor Screening Assay Kit (BPS Cat. #72021) and/or with purified, active IDO1, His-tag (BPS Cat. #71182) in combination with 1x IDO1 Assay Buffer (BPS Cat. #73002).

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MTS-06-C 960/pk
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MTS-11-C 960/pk
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Falcon Round Bottom Polystyrene Tube 5ml With Cap Individual wrap
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MINI TUBE, INDIVIDUAL, POLYPROPYLENE, 0.65 ML, CLEAR, RACKED, STERILE, BULK
MTS-06-C-R-S 960/pk
EUR 517.2
Description: Deep Well/Assay Plates - Axygen; Assay/Other - Axygen

MINI TUBE, INDIVIDUAL, POLYPROPYLENE, 1.1 ML, CLEAR, RACKED, STERILE, BULK
MTS-11-C-R-S 960/pk
EUR 397.2
Description: Deep Well/Assay Plates - Axygen; Assay/Other - Axygen

amfiSure PCR Premix, 8-Strip 0.2ml tube with Individual attached cap
P0316-096 96 rxns
EUR 208.8

amfiSure PCR Premix, 8-Strip 0.2ml tube with Individual attached cap
P0316-480 5X96 rxns
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amfiSure PCR Premix, 8-Strip 0.2ml tube with Individual attached cap
P0316-960 10X96 rxns
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Falcon Round Bottom Polypropylene Tube 14ml With Cap Individual wrap
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MINI TUBE, INDIVIDUAL, POLYPROPYLENE, 0.65 ML, CLEAR, RACKED, NONSTERILE, BULK
MTS-06-C-R 960/pk
EUR 436.8
Description: Deep Well/Assay Plates - Axygen; Assay/Other - Axygen

MINI TUBE, INDIVIDUAL, POLYPROPYLENE, 1.1 ML, CLEAR, RACKED, NONSTERILE, BULK
MTS-11-C-R 960/pk
EUR 336
Description: Deep Well/Assay Plates - Axygen; Assay/Other - Axygen

Microsart Filter Units 100mL 0.45um White CN Membrane w/ Lid Individual Sterile
FIL5636 PK27
EUR 204

Microsart Filter Units 100mL 0.45um Black CN Membrane w/ Lid Individual Sterile
FIL5638 PK27
EUR 204

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FIL5640 PK27
EUR 204

Microsart Filter Units 250mL 0.45um White CN Membrane w/ Lid Individual Sterile
FIL5642 PK18
EUR 145.2

Microsart Filter Units 250mL 0.45um Black CN Membrane w/ Lid Individual Sterile
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EUR 145.2

Microsart Filter Units 250mL 0.2um White CN Membrane w/ Lid Individual Sterile
FIL5646 PK18
EUR 145.2

Syringe Filtration - Aqueous Solutions, Sterile, Individual Pack, 0.22UM, 13mm, 10/Pk
SFA1322S 10UNIT
EUR 81.66

Syringe Filtration - Aqueous Solutions, Sterile, Individual Pack, 0.45UM, 13mm, 10/Pk
SFA1345S 10UNIT
EUR 81.66

Syringe Filtration - Aqueous Solutions, Sterile, Individual Pack, 0.22UM, 25mm, 10/Pk
SFA2522S 10UNIT
EUR 84.31

Syringe Filtration - Aqueous Solutions, Sterile, Individual Pack, 0.45UM, 25mm, 10/Pk
SFA2545S 10UNIT
EUR 84.31

Syringe Filtration - Organic Solutions, Sterile, Individual Pack, 0.22UM, 13mm, 10/Pk
SFO1322S 10UNIT
EUR 83.42

Syringe Filtration - Organic Solutions, Sterile, Individual Pack, 0.45UM, 13mm, 10/Pk
SFO1345S 10UNIT
EUR 83.42

Syringe Filtration - Organic Solutions, Sterile, Individual Pack, 0.22UM, 25mm, 10/Pk
SFO2522S 10UNIT
EUR 86.08

Syringe Filtration - Organic Solutions, Sterile, Individual Pack, 0.45UM, 25mm, 10/Pk
SFO2545S 10UNIT
EUR 86.08

Microsart Filter Units 100mL 0.45um White PVDF Membrane w/ Lid Individual Sterile
FIL5650 PK27
EUR 259.2

Multi Color Puck Option, Individual colors: Red, Black, Blue, Gray, Purple, Green, Orange
M-CP-CustomColors 1 SERVICE Ask for price
Description: Multi Color Puck Option, Individual colors: Red, Black, Blue, Gray, Purple, Green, Orange

Corning 96 Well Polypropylene Cluster Tubes Individual Format 96 Tubes per Rack Sterile
4411 PK960
EUR 267.6

Corning 96 Well Cluster Tubes Individual Tube Format Nonsterile 960 Tubes /bag w/out Rack
4401 PK960
EUR 128.4

amfiEco PCR Master Mix,8-Strip RTU(2X), for 50ul reaction
P0704-096 96 rxns
EUR 174

Human/Mouse/Rabbit Anti-S. Pneumococcal vaccine (Custom serotypes) IgG/IgM ELISA Kit (user specified species & PnS serotypes individual or mix), 96 tests, quantitative
560-400-CUX Custom
EUR 1147.2

SEPTA CAP, INDIVIDUAL, FOR 0.5 ML SEQUENZER TUBES, SILICONE, GREY, NONSTERILE, FOR ABI PRISM 310 SEQUENCER, BULK
AM-SEPTA-310 500/pk
EUR 788.4
Description: Sealing Products; Sealing mats - Axygen

CLUSTER TUBE SYSTEM, 96 WELL, INDIVIDUAL, POLYPROPYLENE, 1.2 ML, NOT TREATED, NO CAPS, RACKED, NONSTERILE, BULK
4410 96/pk
EUR 202.8
Description: Spin-X; Cluster Tubes

2X PCR Pre-Mix (EF) (Reaction Volume 30ul) (96 Tubes)
9K-002-0001 96tubes, 96prep
EUR 373.2

2X PCR Pre-Mix (EF) (Reaction Volume 30ul) (576 Tubes)
9K-002-0002 576tubes, 576prep
EUR 1391.1

2X Reaction Buffer
1068-20 each
EUR 222

2X Reaction Buffer
1068-80 each
EUR 352.8

STORAGE TUBES 96, INDIVIDUAL, POLYPROPYLENE, 1.3 ML, NATURAL, ROUND BOTTOM, NO SCREW CAPS, NONSTERILE, WITH 1D/2D BAR CODE, BULK
8500 96/pk
EUR 685.2
Description: Cryogenic Vials; 2D Barcoded Cryogenic Vials

Pfu 2x Master Mix - 200 Reactions
3326 1/EA
EUR 360

Multiple Tumor Tissue Array - 40 types of tumors. Most of them have duplicates or triplicates from different individual
Z7020082 5 slides
EUR 1657.2
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Reaction Columns 2ml
CHR5348 PK100
EUR 204

TDO Reaction Solution
73005 10 ml
EUR 205
Description: TDO Reaction Solution is an optimized, proprietary formulation containing all the necessary reaction components for TDO-catalyzed conversion of L-tryptophan (L-Trp) to kynurenine (Kyn) in in vitro enzyme activity assays. This product is intended to be used with the BPS TDO Inhibitor Screening Assay Kit (BPS Cat. #72023) and/or with purified, active TDO, His-tag (BPS Cat. #71195) in combination with 1x TDO Assay Buffer (BPS Cat. #73006).

IDO1 Reaction Solution
73001 10 ml
EUR 205
Description: IDO1 Reaction Solution is an optimized, proprietary formulation containing all the necessary reaction components for IDO1-catalyzed conversion of L-tryptophan (L-Trp) to kynurenine (Kyn) in in vitro enzyme activity assays. This product is intended to be used with the BPS IDO1 Inhibitor Screening Assay Kit (BPS Cat. #72021) and/or with purified, active IDO1, His-tag (BPS Cat. #71182) in combination with 1x IDO1 Assay Buffer (BPS Cat. #73002).

IDO2 Reaction Solution
73003 10 ml
EUR 205
Description: IDO2 Reaction Solution is an optimized, proprietary formulation containing all the necessary reaction components for IDO2-catalyzed conversion of L-tryptophan (L-Trp) to kynurenine (Kyn) in in vitro enzyme activity assays. This product is intended to be used with the BPS IDO2 Inhibitor Screening Assay Kit (BPS Cat. #72022) and/or with purified, active IDO2, His-tag (BPS Cat. #71194) in combination with 1x IDO2 Assay Buffer (BPS Cat. #73004).

Reagent Reservoirs 12 Channel Basin, Sterile, Individually wrapped
LC2003-020 20/pk
EUR 343.2

Templiphi 2000 Reaction Kit
28964286 EACH
EUR 4444.8

MyFi Reaction Buffer, 5x
BIO-37115 3 x 1.25ml Ask for price

1mL Pipette Individually Wrapped Plastic
170364N PK1000
EUR 192

2mL Pipette Individually Wrapped Plastic
170365N PK500
EUR 100.8

5mL Pipette Individually Wrapped Plastic
170366N PK200
EUR 62.4

10mL Pipette Individually Wrappd Plastic
170367N PK200
EUR 78

25mL Pipette Individually Wrappd Plastic
170368N PK200
EUR 139.2

50mL Pipette Individually Wrappd Plastic
170369N PK100
EUR 193.2

STORAGE TUBES 96, INDIVIDUAL, POLYPROPYLENE, 1.3 ML, NATURAL, ROUND BOTTOM, WITH ORANGE SCREW CAPS, NONSTERILE, WITH 1D/2D BAR CODE, BULK
8502 96/pk
EUR 943.2
Description: Cryogenic Vials; 2D Barcoded Cryogenic Vials

RANGER Reaction Buffer, 5x
BIO-37114 3 x 1.25ml Ask for price

L shaped cell spreader individually wrapped
10177 PK500
EUR 76.8

Meat sample spatulas (individually wrapped) 100/pk
MSSW-100 1 pk
EUR 73.2

Meat sample spatulas (individually wrapped) 1000/pk
MSSW-1000 1 pk
EUR 182.4

0.2 ml 8-Strip PCR Tube with Individually Attached Caps
GP900 120 strips
EUR 198

CN Membrane 0.2um individually sterile packaged
FIL1240 PK48
EUR 357.6

Reservoir, 25ml sterile, Individually wrapped
P8025-1S 100/pack
EUR 119.94
Description: Reservoir 25ml sterile

Reservoir, 50ml sterile , Individually wrapped
P8050-1S 100/pack
EUR 119.94
Description: Reservoir 50ml sterile

Reservoir, 100ml sterile , Individually wrapped
P8100-1S 100/pack
EUR 128.04
Description: Reservoir 100ml sterile

ReadiUseâ„¢ PCR Reaction Buffer
17202-50mL 50 mL
EUR 95
Description: Our ready to use PCR buffer contains several components essential for the PCR reaction. The PCR reaction buffer helps to maintain a stable pH during the PCR reaction. It also contains a salt that helps to maintain the integrity of the DNA during the reaction and also enhances the activity of the DNA polymerase enzyme.

amfiXpand 2x Reaction Buffer
P0333-001 1ml
EUR 121.2

Hi-Fi Reaction Buffer, 5x
BIO-37110 2 x 1.5ml Ask for price

epTIPS Singles 0.1-20ul Biopur individually wrapped
E0030010019 PK100
EUR 31.46

epTIPS Singles 0.1-20ul Biopur individually wrapped
E0030010027 PK100
EUR 34.8

epTIPS Singles 2-200ul Biopur individually wrapped
E0030010035 PK100
EUR 31.46

epTIPS Singles 2-200ul Biopur individually wrapped
E0030010043 PK100
EUR 34.8

epTIPS Singles 50-1000ul Biopur individually wrapped
E0030010060 PK100
EUR 34.8

amfiFusion 2x Reaction Buffer
P0343-001 1ml
EUR 124.8

illustra Hot Start Master Mix 100 reactions
25150001 EACH
EUR 162

Syringe Ecostep 0.75 mL sterile (individually packaged)
DD985185 PK50
EUR 270

Syringe Ecostep 3.75 mL sterile (individually packaged)
DD985187 PK50
EUR 274.8

Container 180ml Red Cap Individually Wrapped Sterile
CON1029 PK200
EUR 216

Sterilpack D200ST Sterile Individually Packed
F161330 PK400
EUR 87.6

MyTaq Reaction Buffer Red, 5x
BIO-37112 4 x 1ml Ask for price

Accuris qMax Green, No Rox qPCR Mix, 1000 reactions
PR2000-N-1000 1 PC
EUR 623.8

Accuris qMax Probe No Rox qPCR Mix, 1000 reactions
PR2001-N-1000 1 PC
EUR 623.8

Sterilpack D1000ST Sterile Individually Packed
F161340 PK400
EUR 96

Transfer pipette, 4ml, sterile, individually wrapped
P4133-11 500/pack
EUR 108.73
Description: Transfer pipette 4ml

Accuris qMax Green, Low Rox qPCR Mix, 1000 reactions
PR2000-L-1000 1 PC
EUR 623.8

Accuris qMax Probe, Low Rox qPCR Mix, 1000 reactions
PR2001-L-1000 1 PC
EUR 623.8

Eppendorf Tubes 5.0ml Biopur 50 pcs. Individually packed tubes
E0030119479 EACH
EUR 56.28

Cell Strainer, 70 µm, Sterile, Individually Wrapped, Nylon Mesh
LC3069-100 100/CS
EUR 262.8

Cell Strainer, 100 µm, Sterile, Individually Wrapped, Nylon Mesh
LC3070-100 100/CS
EUR 262.8

amfiEco Taq Reaction Buffer(2X)
P0703-001 1ml
EUR 100.8

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