GMP-grade neural progenitor derivation and differentiation from clinical-grade human embryonic stem cells

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GMP-grade neural progenitor derivation and differentiation from clinical-grade human embryonic stem cells

Background: A serious problem for the medical use of human pluripotent stem cells is the event of secure, sturdy and managed differentiation protocols. Adaptation of analysis protocols utilizing reagents designated as research-only to these that are appropriate for medical use, also known as good manufacturing follow (GMP) reagents, is an important and laborious step within the translational pipeline. Nonetheless, revealed protocols to help this course of stay very restricted.

Strategies: We tailored research-grade protocols for the derivation and differentiation of long-term neuroepithelial stem cell progenitors (lt-NES) to GMP-grade reagents and elements appropriate for medical functions. We screened the robustness of the protocol with six clinical-grade hESC traces deposited within the UK Stem Cell Financial institution.

Outcomes: Right here, we current a brand new GMP-compliant protocol to derive lt-NES, that are multipotent, bankable and karyotypically steady. This protocol resulted in sturdy and reproducible differentiation of a number of clinical-grade embryonic stem cells from which we derived lt-NES. Moreover, GMP-derived lt-NES demonstrated a excessive neurogenic potential whereas retaining the flexibility to be redirected to a number of neuronal sub-types.

Conclusions: Total, we report the feasibility of derivation and differentiation of clinical-grade embryonic stem cell traces into lt-NES beneath GMP-compliant circumstances. Our protocols may very well be used as a versatile software to hurry up translation-to-clinic of pluripotent stem cells for a wide range of neurological therapies or regenerative medication research.

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G065-12 200 reactions
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G065-2 200 reactions
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G065-8 200 reactions
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G128 500 ul 10 mM each
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G129 1.0 ml 10 mM each
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G943 250 ul 25 mM each
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G945 1.0 ml 25 mM each
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dNTP Mix
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EUR 201
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EUR 116
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Bone Dissociation System 4 (Osteoblasts), Adult Mouse
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M1040-4
EUR 909

ExoStd? Lyophilized Exosome Standard (100 µg, Human Plasma, 4 vials)
M1041-4
EUR 1137

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M1042-4
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ExoStd? Lyophilized Exosome Standard (100 µg, Human Serum, 4 vials)
M1043-4
EUR 1132

ExoStd? Lyophilized Exosome Standard (30 µg, Human Urine, 4 vials)
M1044-4
EUR 909

ExoStd? Lyophilized Exosome Standard (100 µg, Human Urine, 4 vials)
M1045-4
EUR 1137

ExoStd? Lyophilized Exosome Standard (30 µg, Human Saliva, 4 vials)
M1046-4
EUR 914

ExoStd? Lyophilized Exosome Standard (100 µg, Human Saliva, 4 vials)
M1047-4
EUR 1137

ExoStd? Lyophilized Exosome Standard (30 µg, U87 MG, 4 vials)
M1054-4
EUR 914

ExoStd? Lyophilized Exosome Standard (100 µg, U87 MG, 4 vials)
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ACCUZYME Mix, 2x
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MyTaq Mix, 2x
BIO-25041/S Sample Ask for price

MyTaq Mix, 2x
BIO-25042 1000 Reactions Ask for price

MyFi Mix, 2x
BIO-25049 100 Reactions Ask for price

MyFi Mix, 2x
BIO-25049/S Sample Ask for price

MyFi Mix, 2x
BIO-25050 500 Reactions Ask for price

Ranger Mix, 2x
BIO-25051/S Sample Ask for price

Ranger Mix, 2x
BIO-25052 500 Reactions Ask for price

Glucose Nutrient Mix
109 150 g
EUR 67

Green Taq Mix
P131-01 5 ml
EUR 119

Green Taq Mix
P131-02 15 ml
EUR 150

Green Taq Mix
P131-03 50 ml
EUR 248

Jade? Master Mix
M1105-500
EUR 441

Jade? Mix-iCycler
M1106-500
EUR 441

Jade? Mix-ROX
M1108-500
EUR 441

Jade Express? Mix
M1109-500
EUR 441

Jade Smart? Mix
M1113-500
EUR 441

Jade miRNA? Mix
M1117-500
EUR 441

Taqman Master Mix
M1121-500
EUR 441

Ready? PCR Mix
M1127-1000
EUR 512

Ready? PCR Mix
M1127-200
EUR 191

Rigor? PCR Mix
M1132-200
EUR 321

Breeze? PCR Mix
M1134-200
EUR 267

Distant? PCR Mix
M1136-200
EUR 294

Advance? PCR Mix
M1139-200
EUR 376

Aflatoxin MIX Standard
SD009 0.5ug/mL
EUR 607

dNTP/dUTP Mix
DB2236 1ml
EUR 58.7
  • Product category: PCR Related/dNTPs/dNTP Mix

amfiRivert Reverse Transcriptase Reaction Buffer(5X)
A1203-001 1ml
EUR 88

Adrenal Dissociation System 4 (Heart, Adrenal chromaffin, Paraneurons), Mouse and Rat
4-20204 ea Ask for price

Kidney Dissociation System 4 (Inner medullary duct,Papillae), Mouse and Rat
4-29294 ea Ask for price

ExoStd? Lyophilized Exosome Standard (30 µg, COLO1 cell line, 4 vials)
M1048-4
EUR 914

ExoStd? Lyophilized Exosome Standard (100 µg, COLO1 cell line, 4 vials)
M1049-4
EUR 1142

ExoStd? Lyophilized Exosome Standard (30 µg, MM1 cell line, 4 vials)
M1050-4
EUR 914

ExoStd? Lyophilized Exosome Standard (100 µg, MM1 cell line, 4 vials)
M1051-4
EUR 1142

ExoStd? Lyophilized Exosome Standard (30 µg, BLCL21 cell line, 4 vials)
M1052-4
EUR 914

ExoStd? Lyophilized Exosome Standard (100 µg, BLCL21 cell line, 4 vials)
M1053-4
EUR 1142

ExoStd? Lyophilized Exosome Standard (30 µg, HCT116 cell line, 4 vials)
M1058-4
EUR 914

ExoStd? Lyophilized Exosome Standard (100 µg, HCT116 cell line, 4 vials)
M1059-4
EUR 1142

ExoStd? Lyophilized Exosome Standard (30 µg, PC3 cell line, 4 vials)
M1060-4
EUR 914

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