Human ALCAM PicoKine ELISA Kit

Description: A sandwich quantitative ELISA assay kit for detection of Mouse Activated Leukocyte Cell Adhesion Molecule (ALCAM) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Description: A sandwich quantitative ELISA assay kit for detection of Mouse Activated Leukocyte Cell Adhesion Molecule (ALCAM) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Description: A sandwich quantitative ELISA assay kit for detection of Rat Activated Leukocyte Cell Adhesion Molecule (ALCAM) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Description: A sandwich quantitative ELISA assay kit for detection of Rat Activated Leukocyte Cell Adhesion Molecule (ALCAM) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Description: ELISA Kit for detection of ALCAM in the research laboratory

Description: Description of target: CD166 antigen is a 100-105 kD typeI transmembrane glycoprotein that is a member of the immunoglobulin superfamily of proteins. In humans it is encoded by the ALCAM gene. The gene was mapped to human chromosome 3q13.1-q13.2 by fluorescence in situ hybridization of cDNA probes to metaphase chromosomes. It is also called CD166 (cluster of differentiation 166), MEMD, SC-1/DM-GRASP/BEN in the chicken, and KG-CAM in the rat. It is expressed on activated T cells, activated monocytes, epithelial cells, fibroblasts, neurons, melanoma cells, and also in sweat and sebaceous glands. CD166 protein expression is reported to be upregulated in a cell line deriving from a metastasizing melanoma. CD166 plays an important role in mediating adhesion interactions between thymic epithelial cells and CD6+ cells during intrathymic T cell development. Recently, CD166 has also been used as a potential cancer stem cell marker.;Species reactivity: Human;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich Immunoassay;Sensitivity: <= 10 pg/mL

Description: Enzyme-linked immunosorbent assay kit for quantification of Human ALCAM in samples from serum, plasma, tissue homogenates and other biological fluids.

Description: Description of target: Cell adhesion molecule that mediates both heterotypic cell-cell contacts via its interaction with CD6, as well as homotypic cell-cell contacts (PubMed:7760007, PubMed:15496415, PubMed:15048703, PubMed:16352806, PubMed:23169771, PubMed:24945728). Promotes T-cell activation and proliferation via its interactions with CD6 (PubMed:15048703, PubMed:16352806, PubMed:24945728). Contributes to the formation and maturation of the immunological synapse via its interactions with CD6 (PubMed:15294938, PubMed:16352806). Mediates homotypic interactions with cells that express ALCAM (PubMed:15496415, PubMed:16352806). Required for normal hematopoietic stem cell engraftment in the bone marrow (PubMed:24740813). Mediates attachment of dendritic cells onto endothelial cells via homotypic interaction (PubMed:23169771). Inhibits endothelial cell migration and promotes endothelial tube formation via homotypic interactions (PubMed:15496415, PubMed:23169771). Required for normal organization of the lymph vessel network. Required for normal hematopoietic stem cell engraftment in the bone marrow. Plays a role in hematopoiesis; required for normal numbers of hematopoietic stem cells in bone marrow. Promotes in vitro osteoblast proliferation and differentiation (By similarity). Promotes neurite extension, axon growth and axon guidance; axons grow preferentially on surfaces that contain ALCAM. Mediates outgrowth and pathfinding for retinal ganglion cell axons (By similarity).By similarity <p>Manually curated information which has been propagated from a related experimentally characterized protein.</p> <p><a href="/manual/evidences#ECO:0000250">More…</a></p> Manual assertion inferred from sequence similarity toiUniProtKB:P42292 (CD166_CHICK)6 Publications <p>Manually curated information for which there is published experimental evidence.</p> <p><a href="/manual/evidences#ECO:0000269">More…</a></p> Manual assertion based on experiment iniRef.1"Cloning, mapping, and characterization of activated leukocyte-cell adhesion molecule (ALCAM), a CD6 ligand."_x005F_x005F_x000D_Bowen M.A., Patel D.D., Li X., Modrell B., Malacko A.R., Wang W.-C., Marquardt H., Neubauer M., Pesando J.M., Francke U., Haynes B.F., Aruffo A._x005F_x005F_x000D_J. Exp. Med. 181:2213-2220(1995) [PubMed] [Europe PMC] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANT MET-301, PARTIAL PROTEIN SEQUENCE, INTERACTION WITH CD6, SUBUNIT, SUBCELLULAR LOCATION, FUNCTION, TISSUE SPECIFICITY.Ref.2"Molecular isolation and characterization of a soluble isoform of activated leukocyte cell adhesion molecule that modulates endothelial cell function."_x005F_x005F_x000D_Ikeda K., Quertermous T._x005F_x005F_x000D_J. Biol. Chem. 279:55315-55323(2004) [PubMed] [Europe PMC] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3), SUBCELLULAR LOCATION (ISOFORM 3), FUNCTION (ISOFORMS 1 AND 3), GLYCOSYLATION, TISSUE SPECIFICITY.Ref.10"Frontline: Optimal T cell activation requires the engagement of CD6 and CD166."_x005F_x005F_x000D_Hassan N.J., Barclay A.N., Brown M.H._x005F_x005F_x000D_Eur. J. Immunol. 34:930-940(2004) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION, INTERACTION WITH CD6, SUBUNIT, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.Ref.11"Relevance of CD6-mediated interactions in T cell activation and proliferation."_x005F_x005F_x000D_Gimferrer I., Calvo M., Mittelbrunn M., Farnos M., Sarrias M.R., Enrich C., Vives J., Sanchez-Madrid F., Lozano F._x005F_x005F_x000D_J. Immunol. 173:2262-2270(2004) [PubMed] [Europe PMC] [Abstract]Cited for: SUBCELLULAR LOCATION.Ref.13"Long-term engagement of CD6 and ALCAM is essential for T-cell proliferation induced by dendritic cells."_x005F_x005F_x000D_Zimmerman A.W., Joosten B., Torensma R., Parnes J.R., van Leeuwen F.N., Figdor C.G._x005F_x005F_x000D_Blood 107:3212-3220(2006) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH CD6, SUBUNIT, TISSUE SPECIFICITY.Ref.20"Modulation of CD6 function through interaction with galectin-1 and -3."_x005F_x005F_x000D_Escoda-Ferran C., Carrasco E., Caballero-Banos M., Miro-Julia C., Martinez-Florensa M., Consuegra-Fernandez M., Martinez V.G., Liu F.T., Lozano F._x005F_x005F_x000D_FEBS Lett. 588:2805-2813(2014) [PubMed] [Europe PMC] [Abstract]Cited for: INTERACTION WITH CD6; LGALS1 AND LGALS3, GLYCOSYLATION, FUNCTION, SUBCELLULAR LOCATION. Isoform 3: Inhibits activities of membrane-bound isoforms by competing for the same interaction partners. Inhibits cell attachment via homotypic interactions. Promotes endothelial cell migration. Inhibits endothelial cell tube formation.1 Publication <p>Manually curated information for which there is published experimental evidence.</p> <p><a href="/manual/evidences#ECO:0000269">More…</a></p> Manual assertion based on experiment iniRef.2"Molecular isolation and characterization of a soluble isoform of activated leukocyte cell adhesion molecule that modulates endothelial cell function."_x005F_x005F_x000D_Ikeda K., Quertermous T._x005F_x005F_x000D_J. Biol. Chem. 279:55315-55323(2004) [PubMed] [Europe PMC] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3), SUBCELLULAR LOCATION (ISOFORM 3), FUNCTION (ISOFORMS 1 AND 3), GLYCOSYLATION, TISSUE SPECIFICITY. ;Species reactivity: Human;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich Immunoassay;Sensitivity: < 0.068 ng/mL